Evidence map›Paper›PMID 34228783›Full record

ArticleThe Journal of cell biology2021

Label-retention expansion microscopy.

Xiaoyu Shi, Qi Li, Zhipeng Dai, Arthur A Tran, Siyu Feng, Alejandro D Ramirez, Zixi Lin, Xiaomeng Wang, Tracy T Chow, Jiapei Chen and 6 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
14.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 81 citations in OpenAlex.

  1. Years-long stability of X10 expansion microscopy hydrogels.Journal of biological engineering · 2026
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  4. Organization of Myosin H in the Apical Complex ofbioRxiv : the preprint server for biology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Xiaoyu Shi *Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.ORCID 0000-0002-9634-2659
Qi Li *Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.ORCID 0000-0002-6891-5923
Zhipeng DaiDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA.
Arthur A TranGraduate Program in Chemistry and Chemical Biology, University of California, San Francisco, San Francisco, CA.
Siyu FengUniversity of California, Berkeley-University of California, San Francisco Joint Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA.
Alejandro D RamirezDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Zixi LinDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Xiaomeng WangDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Tracy T ChowDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.
Jiapei ChenDepartment of Pathology, University of California, San Francisco, San Francisco, CA.
Dhivya KumarDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.ORCID 0000-0002-3737-014X
Andrew R McCollochDepartment of Developmental and Cell Biology, University of California, Irvine, Irvine, CA.ORCID 0000-0003-3256-5826
Jeremy F ReiterCardiovascular Research Institute, University of California, San Francisco, San Francisco, CA.ORCID 0000-0002-6512-320X
Eric J HuangDepartment of Pathology, University of California, San Francisco, San Francisco, CA.ORCID 0000-0002-5381-3801
Ian B SeipleDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.
Bo HuangDepartment of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA.ORCID 0000-0003-1704-4141
University of California, San Francisco · USUniversity of California, Irvine · US

Funding

Solving macromolecular complex architecture in situ by super-resolution microscopDP2OD008479 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2011 to 2011
$2.3M
Transition zone control of ciliary signalingR01DE029454 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI REITER, JEREMY F · 2019 to 2022
$2.3M
Structure mapping of molecular complexes by super-resolution microscopyR01GM124334 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, BO · 2018 to 2021
$1.3M
Elucidating How Primary Cilia Regulate Hedgehog Signaling by Super-Resolution MicroscopyR00GM126136 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI SHI, XIAOYU · 2020 to 2022
$746k
Elucidating How Primary Cilia Regulate Hedgehog Signaling by Super-Resolution MicroscopyK99GM126136 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHI, XIAOYU · 2018 to 2019
$128k
American Heart Association-American Stroke Association 19PRE3480616NIDCR NIH HHS R01 DE029454NIGMS NIH HHS K99 GM126136NIGMS NIH HHS R00 GM126136NIGMS NIH HHS R01 GM124334NIH HHS DP2 OD008479NIH HHS DP2OD008479
6 · The paper itself

Abstract

Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.

Indexed as

AnimalsAntibodiesBiotinCell Line, TumorFluorescent DyesHEK293 CellsHeLa CellsHumansMiceMicroscopy, FluorescenceMicrotubulesMouse Embryonic Stem CellsOsteoblastsStaining and LabelingStreptavidinSuccinimidesAntibodiesBiotinFluorescent DyesN-hydroxysuccinimideStreptavidinSuccinimides

Identifiers

PMID34228783
PMCPMC8266563
OpenAlexW3178364274

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.