ArticlePloS one2021
Rapid diagnosis and reduced workload for urinary tract infection using flowcytometry combined with direct antibiotic susceptibility testing.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- The evidence landscape for direct-from-urine antibiotic susceptibility testing (AST) for urinary tract infection (UTI): a scoping review.JAC-antimicrobial resistance · 2026Review
- Diagnostic Accuracy of Urine Flow Cytometry (UF) in Urinary Tract Infection (UTI) Detection and Management: A Systematic Review and Meta-Analysis.Diagnostics (Basel, Switzerland) · 2026Review
- Quality indicators for urine sample contamination: can squamous epithelial cells and bacteria count be used to identify properly collected samples?Clinical chemistry and laboratory medicine · 2025Observational
- Using the Sysmex UF-4000 urine flow cytometer for rapid diagnosis of urinary tract infection in the clinical microbiological laboratory.Journal of clinical laboratory analysis · 2024Article
- [Evaluation of Sysmex UF-5000 flow cytometer flag BACT-info for Gram discrimination in urinary tract infection].Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2024Article
- Enhancing clinical decision-making: Sysmex UF-5000 as a screening tool for bacterial urinary tract infection in children.PloS one · 2024Article
- Analysis of factors with low positive predictive value in the diagnosis of urinary tract infection by flow cytometry.World journal of urology · 2023Article
- Accuracy of the Sysmex UF-5000 analyzer for urinary tract infection screening and pathogen classification.PloS one · 2023Article
- Urine Flow Cytometry Parameter Cannot Safely Predict Contamination of Urine-A Cohort Study of a Swiss Emergency Department Using Machine Learning Techniques.Diagnostics (Basel, Switzerland) · 2022Article
- A novel approach to screening and managing the urinary tract infections suspected sample in the general human population.Frontiers in cellular and infection microbiology · 2022Article
- Comparison of Laboratory Diagnosis of Urinary Tract Infections Based on Leukocyte and Bacterial Parameters Using Standardized Microscopic and Flow Cytometry Methods.International journal of nephrology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe evaluated if flowcytometry, using Sysmex UF-5000, could improve diagnosis of urinary tract infections by rapid identification of culture negative and contaminated samples prior to culture plating, thus reducing culture plating workload and response time. We also evaluated if it is possible to reduce the response time for antibiotic susceptibility profiles using the bacteria information flag on Sysmex UF-5000 to differentiate between Gram positive and negative bacteria, followed by direct Antibiotic Susceptibility Testing (dAST) on the positive urine samples.
methodsOne thousand urine samples were analyzed for bacteria, white blood cells and squamous cells by flowcytometry before culture plating. Results from flowcytometric analysis at different cut-off values were compared to results of culture plating. We evaluated dAST on 100 urine samples that were analyzed as positive by flowcytometry, containing either Gram positive or Gram negative bacteria.
resultsUsing a cut-off value with bacterial count ≥100.000/mL and WBCs ≥10/μL, flowcytometry predicted 42,1% of samples with non-significant growth. We found that most contaminated samples contain few squamous cells. For 52/56 positive samples containing Gram negative bacteria dAST was identical to routine testing. Overall, there was concordance in 555/560 tested antibiotic combinations.
conclusionFlowcytometry offers advantages for diagnosis of urinary tract infections. Screening for negative urine samples on the day of arrival reduces culture plating and workload, and results in shorter response time for the negative samples. The bacteria information flag predicts positive samples containing Gram negative bacteria for dAST with high accuracy, thus Antibiotic Susceptibility Profile can be reported the day after arrival. For the positive samples containing Gram negative bacteria the concordance was very good between dAST and Antibiotic Susceptibility Testing in routine. For positive samples containing Gram positive bacteria the results were not convincing. We did not find any correlation between epithelial cells and contamination.
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