ArticlePLoS genetics2021
CRISPR/Cas9 modified An. gambiae carrying kdr mutation L1014F functionally validate its contribution in insecticide resistance and combined effect with metabolic enzymes.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 47 citations in OpenAlex.
- Synergistic action of different molecular mechanisms causes striking levels of insecticide resistance in the malaria vectorProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.BMC genomics · 2026Article
- On the use of generative models for demographic inference in malaria vectors from genomic data.G3 (Bethesda, Md.) · 2026Article
- Insecticide resistance level and geographical distribution of target-site mutations in field collections of the house fly (Musca domestica) from Türkiye.Ecotoxicology (London, England) · 2026Article
- Larval habitat drives differential selection pressures on genetic insecticide resistance and metabolic enzyme plasticity in Anopheles gambiae s.l during laboratory colonization.Scientific reports · 2026Article
- Multimodal platform for ITN efficacy: Surface chemistry, bioavailability, and mosquito behavior.Science advances · 2026Article
- Genetic Surveillance Reveals Differential Evolutionary Dynamic of Anopheles gambiae Under Contrasting Insecticidal Tools Used in Malaria Control.Molecular ecology · 2026Article
- First Detection of the L1014F kdr Mutation in Triatoma dimidiata, a Vector of Chagas Disease in Mexico.Neotropical entomology · 2026Article
- Predictive chemoproteomics and functional validation reveal Coeae6g-mediated insecticide cross-resistance in the malaria vector Anopheles gambiae.Nature communications · 2025Article
- Article
- An atlas of positive selection in the genomes of major malaria vectors.bioRxiv : the preprint server for biology · 2025Article
- CRISPR/Cas Technology in Insect Insecticide Resistance.Insects · 2025Review
- Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.International journal of molecular sciences · 2025Review
- Insecticide resistant Anopheles from Ethiopia but not Burkina Faso show a microbiota composition shift upon insecticide exposure.Parasites & vectors · 2025Article
- Genome-wide association studies unveil major genetic loci driving insecticide resistance in Anopheles funestus in four eco-geographical settings across Cameroon.BMC genomics · 2024Article
- A self-eliminating allelic-drive reverses insecticide resistance in Drosophila leaving no transgene in the population.Nature communications · 2024Article
- Discovery of Knock-Down Resistance in the Major African Malaria Vector Anopheles funestus.Molecular ecology · 2024Article
- Longitudinal survey of insecticide resistance in a village of central region of Burkina Faso reveals co-occurrence of 1014F, 1014S and 402L mutations in Anopheles coluzzii and Anopheles arabiensis.Malaria journal · 2024Article
- Discovery of knock-down resistance in the major African malaria vectorbioRxiv : the preprint server for biology · 2024Article
- Making genome editing a success story in Africa.Nature biotechnology · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
Insecticide resistance in Anopheles mosquitoes is a major obstacle in maintaining the momentum in reducing the malaria burden; mitigating strategies require improved understanding of the underlying mechanisms. Mutations in the target site of insecticides (the voltage gated sodium channel for the most widely used pyrethroid class) and over-expression of detoxification enzymes are commonly reported, but their relative contribution to phenotypic resistance remain poorly understood. Here we present a genome editing pipeline to introduce single nucleotide polymorphisms in An. gambiae which we have used to study the effect of the classical kdr mutation L1014F (L995F based on An. gambiae numbering), one of the most widely distributed resistance alleles. Introduction of 1014F in an otherwise fully susceptible genetic background increased levels of resistance to all tested pyrethroids and DDT ranging from 9.9-fold for permethrin to >24-fold for DDT. The introduction of the 1014F allele was sufficient to reduce mortality of mosquitoes after exposure to deltamethrin treated bednets, even as the only resistance mechanism present. When 1014F was combined with over-expression of glutathione transferase Gste2, resistance to permethrin increased further demonstrating the critical combined effect between target site resistance and detoxification enzymes in vivo. We also show that mosquitoes carrying the 1014F allele in homozygosity showed fitness disadvantages including increased mortality at the larval stage and a reduction in fecundity and adult longevity, which can have consequences for the strength of selection that will apply to this allele in the field.
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