ArticleeLife2021
Dynamics of primitive streak regression controls the fate of neuromesodermal progenitors in the chicken embryo.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Reciprocal coupling between EMT and BMP morphogen signalling drives collective cell invasion.The EMBO journal · 2026Article
- Single-cell mapping of homocysteine-induced perturbations in avian embryogenesis using LMO and BD rhapsody transcriptomics.iScience · 2026Article
- Brachyury expression levels predict lineage potential and axis-forming ability of in vitro-derived neuromesodermal progenitors.PLoS biology · 2026Article
- Secondary neurulation mechanisms likely arose in vertebrates during mesenchymal tailbud extension.Frontiers in cell and developmental biology · 2026Article
- Tissue fluidity mediates a trade-off between the speed and accuracy of multicellular patterning by cell sorting.Biophysical journal · 2025Article
- The ratio of Wnt signaling activity to Sox2 transcription factor levels predicts neuromesodermal fate potential.Development (Cambridge, England) · 2025Article
- Tissue Fluidity Mediates a Trade-off Between the Speed and Accuracy of Multicellular Patterning by Cell Sorting.bioRxiv : the preprint server for biology · 2025Article
- A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations.PLoS biology · 2025Article
- Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates.FASEB bioAdvances · 2025Article
- Dual genetic tracing demonstrates the heterogeneous differentiation and function of neuromesodermal progenitors in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- An in vivo CRISPR screen in chick embryos reveals a role for MLLT3 in specification of neural cells from the caudal epiblast.Development (Cambridge, England) · 2025Article
- Timely TGFβ signalling inhibition induces notochord.Nature · 2025Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
- Cellular and molecular control of vertebrate somitogenesis.Nature reviews. Molecular cell biology · 2024Review
- Differential proliferation regulates multi-tissue morphogenesis during embryonic axial extension: integrating viscous modeling and experimental approaches.Development (Cambridge, England) · 2024Article
- Reconstructing axial progenitor field dynamics in mouse stem cell-derived embryoids.Developmental cell · 2024Article
- Adaptive tail-length evolution in deer mice is associated with differential Hoxd13 expression in early development.Nature ecology & evolution · 2024Article
- Review
- Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors.Development (Cambridge, England) · 2024Article
- Gastrulation: Its Principles and Variations.Results and problems in cell differentiation · 2024Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
In classical descriptions of vertebrate development, the segregation of the three embryonic germ layers completes by the end of gastrulation. Body formation then proceeds in a head to tail fashion by progressive deposition of lineage-committed progenitors during regression of the primitive streak (PS) and tail bud (TB). The identification by retrospective clonal analysis of a population of neuromesodermal progenitors (NMPs) contributing to both musculoskeletal precursors (paraxial mesoderm) and spinal cord during axis formation challenged these notions. However, classical fate mapping studies of the PS region in amniotes have so far failed to provide direct evidence for such bipotential cells at the single-cell level. Here, using lineage tracing and single-cell RNA sequencing in the chicken embryo, we identify a resident cell population of the anterior PS epiblast, which contributes to neural and mesodermal lineages in trunk and tail. These cells initially behave as monopotent progenitors as classically described and only acquire a bipotential fate later, in more posterior regions. We show that NMPs exhibit a conserved transcriptomic signature during axis elongation but lose their epithelial characteristicsin the TB. Posterior to anterior gradients of convergence speed and ingression along the PS lead to asymmetric exhaustion of PS mesodermal precursor territories. Through limited ingression and increased proliferation, NMPs are maintained and amplified as a cell population which constitute the main progenitors in the TB. Together, our studies provide a novel understanding of the PS and TB contribution through the NMPs to the formation of the body of amniote embryos.
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