[Preparation of luminescent silica nanoparticles with immobilized metal ion affinity for labeling phosphorylated proteins in Western Blot].

Yuxiao Mao, Mengmeng Zheng, Guizhen Liu, Baoli An, Jingwu Kang

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Article in Se pu = Chinese journal of chromatography, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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5 authors.

Yuxiao MaoDepartment of Chemistry, College of Science, Shanghai University, Shanghai 200444, China.

Mengmeng ZhengState Key Laboratory of Bioorganic Chemistry and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

Guizhen LiuState Key Laboratory of Bioorganic Chemistry and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

Baoli AnDepartment of Chemistry, College of Science, Shanghai University, Shanghai 200444, China.

Jingwu KangState Key Laboratory of Bioorganic Chemistry and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein phosphorylation is an important type of post-translational protein modification. In Western Blot experiment, the assay of phosphoproteins need special phospho antibodies, which are expensive, difficult to preserve, poorly reproducible. To this end, the immobilized metal ion affinity luminescent silica nanoparticles for instead of phospho antibodies were prepared. A layer of polymer was created on the surface of the silica nanoparticles via co-polymerization to protect the nanoparticles and to functionalize them with the immobilized metal ion affinity property to specifically label the phosphorylated proteins in Western Blot assays. The affinity luminescent silica nanoparticles were prepared with the following procedure. First, the sol-gel precursor fluorescein isothiocyanate-3-aminopropyltriethoxysilane (FITC-APTES) with the fluorescent moiety was prepared by modifying APTES with FITC. The luminescent silica nanoparticles (FITC@SiO

Indexed as

Blotting, WesternNanoparticlesSilicon DioxideAnimalsCattleIonsPhosphorylationProto-Oncogene Proteins A-rafSerum Albumin, BovineTitaniumIonsProto-Oncogene Proteins A-rafSerum Albumin, BovineSilicon DioxideTitaniumimmobilized metal ion affinityluminescent silica nanoparticlesphosphorylated protein labellingWestern Blot

Identifiers

PMID34227758

DOI10.3724/SP.J.1123.2020.05024

PMCPMC9404030

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