Evidence map›Paper›PMID 34226955›Full record

ArticleMikrochimica acta2021

An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.

Lin Ding, Payar Radfar, Meysam Rezaei, Majid Ebrahimi Warkiani

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Lin Ding *School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.
Payar Radfar *School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.
Meysam RezaeiSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia.
Majid Ebrahimi WarkianiSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, 2007, Australia. majid.warkiani@uts.edu.au.
Southern University of Science and Technology · CNUniversity of Technology Sydney · AU

Funding

Australian Research Council DP170103704Australian Research Council DP180103003National Health and Medical Research Council APP1143377
6 · The paper itself

Abstract

In-depth study of cellular heterogeneity of rare cells (e.g. circulating tumour cells (CTCs) and circulating foetal cells (CFCs)) is greatly needed in disease management but has never been completely explored due to the current technological limitations. We have developed a retrieval method for single-cell detection using a static droplet array (SDA) device through liquid segmentation with almost no sample loss. We explored the potential of using SDA for low sample input and retrieving the cells of interest using everyday laboratory equipment for downstream molecular analysis. This single-cell isolation and retrieval method is low-cost, rapid and provides a solution to the remaining challenge for single rare cell detection. The entire process takes less than 15 min, is easy to fabricate and allows for on-chip analysis of cells in nanolitre droplets and retrieval of desired droplets. To validate the applicability of our device and method, we mimicked detection of single CTCs by isolating and retrieving single cells and perform real-time PCR on their mRNA contents.

Indexed as

Biosensing TechniquesCell SeparationHumansLab-On-A-Chip DevicesMCF-7 CellsMicrofluidic Analytical TechniquesMicrofluidicsNeoplastic Cells, CirculatingPolymerase Chain ReactionSingle-Cell AnalysisTHP-1 CellsCancerCellular heterogeneityCirculating tumour cellsDroplet microfluidicsMicrofluidic systemSingle cell detection

Identifiers

PMID34226955
OpenAlexW3182655427

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.