ArticleJournal of microbiology and biotechnology2021
Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells.
Article in Journal of microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 39 citations in OpenAlex.
- Perindopril and L‑arginine protect against arsenic trioxide-induced neurotoxicity via Keap1/Nrf2/HO‑1 activation and suppression of neuroinflammation and apoptosis.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- From By-Product to Bioactive: New Antioxidant and Bioavailable Peptides Derived from Milk Permeate Targeting the Nrf2/Keap1 Pathway in Intestinal Cell Models.Antioxidants (Basel, Switzerland) · 2026Article
- Effect of β-Casein Fortification in Milk Protein on Digestion Properties and Release of Bioactive Peptides in a Suckling Rat Pup Model.Foods (Basel, Switzerland) · 2025Article
- Double-edged sword of L-arginine in diabetes: Exploring anti-inflammatory and antioxidant strategies.World journal of diabetes · 2025Article
- Preparation and characterization of immunopeptides isolated from pig spleen and evaluation of their immunomodulatory propertiesFrontiers in immunology · 2025Article
- Characterization and molecular docking of tetrapeptides with cellular antioxidant and ACE inhibitory properties from cricket (Heliyon · 2024Article
- Preparation, Isolation and Antioxidant Function of Peptides from a New Resource ofFoods (Basel, Switzerland) · 2024Article
- An insight into role of amino acids as antioxidants via NRF2 activation.Amino acids · 2024Review
- Nano casein-pectin complex: exploring physicochemical, organoleptic properties, and LAB viability in skimmed milk and low-fat yoghurt.Frontiers in nutrition · 2023Article
- Targeting Nrf2 with Probiotics and Postbiotics in the Treatment of Periodontitis.Biomolecules · 2022Review
- Silencing MR-1 Protects against Myocardial Injury Induced by Chronic Intermittent Hypoxia by Targeting Nrf2 through Antioxidant Stress and Anti-Inflammation Pathways.Journal of healthcare engineering · 2022Article
- Based on Activation of p62-Keap1-Nrf2 Pathway, Hesperidin Protects Arsenic-Trioxide-Induced Cardiotoxicity in Mice.Frontiers in pharmacology · 2021Article
- Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Casein-derived antioxidant peptides by using microbial proteases have gained increasing attention. Combination of two microbial proteases, Protin SD-NY10 and Protease A "Amano" 2SD, was employed to hydrolyze casein to obtain potential antioxidant peptides that were identified by LCMS/ MS, chemically synthesized and characterized in a oxidatively damaged HepG2 cell model. Four peptides, YQLD, FSDIPNPIGSEN, FSDIPNPIGSE, YFYP were found to possess high 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability. Evaluation with HepG2 cells showed that the 4 peptides at low concentrations (< 1.0 mg/ml) protected the cells against oxidative damage. The 4 peptides exhibited different levels of antioxidant activity by stimulating mRNA and protein expression of the antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), as well as nuclear factor erythroid-2-related factor 2 (Nrf2), but decreasing the mRNA expression of Kelch-like ECH-associated protein 1 (Keap1). Furthermore, these peptides decreased production of reactive oxygen species (ROS) and malondialdehyde (MDA), but increased glutathione (GSH) production in HepG2 cells. Therefore, the 4 casein-derived peptides obtained by using microbial proteases exhibited different antioxidant activity by activating the Keap1-Nrf2 signaling pathway, and they could serve as potential antioxidant agents in functional foods or pharmaceutic preparation.
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