Evidence map›Paper›PMID 34226404›Full record

ArticleJournal of microbiology and biotechnology2021

Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair.

Hyeok-Jin Ko, Heesang Song, In-Geol Choi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Hyeok-Jin KoFood Biotech R&D Center, Samyang Corp., Seongnam 13488, Republic of Korea.
Heesang SongDepartment of Biochemistry and Molecular Biology, Chosun University School of Medicine, Gwangju 61452, Republic of Korea.
In-Geol ChoiDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Chosun University · KRKorea University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a noncovalent protein-protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from

Indexed as

Amino Acid SequenceArchaeal ProteinsArchaeoglobus fulgidusCell Cycle ProteinsCell MembraneCell Surface Display TechniquesChromosomal Proteins, Non-HistoneCohesinsDisaccharidasesEscherichia coliLuminescent ProteinsProtein BindingProtein FoldingRecombinant Fusion ProteinsRed Fluorescent ProteinArchaeal ProteinsCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsDisaccharidasesLuminescent Proteinsneoagarobiose hydrolaseRecombinant Fusion ProteinsRed Fluorescent Proteincohesin–dockerinEscherichia coli cell surface attachmentnon-covalent interaction moduleα-neoagarobiose hydrolase

Identifiers

PMID34226404
PMCPMC9705933
OpenAlexW3180001721

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.