Evidence map›Paper›PMID 34225911›Full record

ArticleMethods in cell biology2021

Quantifying phagosomal HOCl at single immune-cell resolution.

Palapuravan Anees, Matthew Zajac, Yamuna Krishnan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Palapuravan AneesDepartment of Chemistry, University of Chicago, Chicago, IL, United States; Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, University of Chicago, Chicago, IL, United States.
Matthew ZajacDepartment of Chemistry, University of Chicago, Chicago, IL, United States; Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, University of Chicago, Chicago, IL, United States.
Yamuna KrishnanDepartment of Chemistry, University of Chicago, Chicago, IL, United States; Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, University of Chicago, Chicago, IL, United States. Electronic address: yamuna@uchicago.edu.
University of Chicago · US

Funding

ULTRASTRUCTURE AND CYTOMORPHOLOGY COREP30DK042086 · NIDDK · UNIVERSITY OF CHICAGO · PI LIU, CAMBRIAN YANGSHAO · 1990 to 2025
$29.8M
Probing short and long term consequences of Small and Large Bowel Microbiota Transplants on Host Physiology: Implications for the development of future live biotherapeuticsR01DK138072 · NIDDK · UNIVERSITY OF CHICAGO · PI EUGENE B CHANG, Kristina Brooke Martinez-Guryn · 2024 to 2026
$2.1M
Calcium homeostasis in organellesR01NS112139 · NINDS · UNIVERSITY OF CHICAGO · PI KRISHNAN, YAMUNA · 2020 to 2024
$1.8M
A mechanism of lysosomal Calcium entryR21NS114428 · NINDS · UNIVERSITY OF CHICAGO · PI KRISHNAN, YAMUNA · 2019 to 2020
$408k
NIDDK NIH HHS P30 DK042086NIDDK NIH HHS R01 DK138072NINDS NIH HHS R01 NS112139NINDS NIH HHS R21 NS114428
6 · The paper itself

Abstract

Neutralization of pathogens by phagocytic immune cells requires the biogenesis of a compartmentalized hotspot of reactive species called the phagosome. One of these reactive species is hypochlorous acid (HOCl), produced by the enzyme myeloperoxidase (MPO) after the phagosome fuses with the lysosome. Mapping HOCl during phagosome maturation can report on pathogen killing and offer insights into regulation of MPO activity, mechanisms of resistance and host-pathogen interactions. However, this has been difficult because of a lack of a suitable method to chemically map a transient organelle with pH fluctuations like the phagosome. Here, we detail a protocol for quantifying HOCl dynamics in phagosomes using a fluorescent DNA-based reporter. Compared to traditional methods of visualizing HOCl or measuring MPO activity, this method offers sub-cellular spatial resolution and the capacity to assay HOCl production with single cell resolution.

Indexed as

Hypochlorous AcidNeutrophilsPeroxidasePhagocytesPhagosomesHypochlorous AcidPeroxidaseDNA-based reporterFluorescent probeHypochlorous acidImmune cellsMyeloperoxidasepHPhagosome

Identifiers

PMID34225911
PMCPMC11026105
OpenAlexW3099968772

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.