Evidence map›Paper›PMID 34225362›Full record

ArticleBiology of reproduction2021

RNF8 is not required for histone-to-protamine exchange in spermiogenesis†.

Hironori Abe, Rajyalakshmi Meduri, Ziwei Li, Paul R Andreassen, Satoshi H Namekawa

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Hironori AbeDivision of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Rajyalakshmi MeduriDepartment of Biochemistry and Biophysics, Department of Urology, University of Rochester Medical Center, New York, USA.
Ziwei LiDepartment of Biochemistry and Biophysics, Department of Urology, University of Rochester Medical Center, New York, USA.
Paul R AndreassenDivision of Experimental Hematology & Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Satoshi H NamekawaDivision of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Cincinnati Children's Hospital Medical Center · USUniversity of Rochester Medical Center · USUniversity of California, Los Angeles · US

Funding

DNA Damage Response Pathways in Meiotic Sex Chromosome InactivationR01GM098605 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI NAMEKAWA, SATOSHI · 2011 to 2020
$3.3M
Functional characterization of the role of distinct domains of ATM and the impact of sequence variants on the DNA damage responseR01GM134731 · NIGMS · CINCINNATI CHILDRENS HOSP MED CTR · PI ANDREASSEN, PAUL R · 2019 to 2022
$1.3M
NIGMS NIH HHS R01 GM098605NIGMS NIH HHS R01 GM134731
6 · The paper itself

Abstract

While an E3 ubiquitin ligase, RNF8, was initially reported to be required for histone-to-protamine exchange in spermiogenesis, we subsequently demonstrated that RNF8 is not involved in this process. Nevertheless, reflecting a lingering misunderstanding in the field, a growing number of studies have continued to postulate a requirement for RNF8 in the histone-to-protamine exchange. For example, a recent study claimed that a mouse PIWI protein, MIWI, controls RNF8-mediated histone-to-protamine exchange. Here, confirming our earlier conclusions, we show that RNF8 is required neither for the establishment of histone H4K16 acetylation, which is an initial step in histone removal during spermiogenesis, nor for the incorporation of two protamine proteins, PRM1 and PRM2. Thus, whereas RNF8 mediates ubiquitination of H2A on the sex chromosomes in meiosis, during the prior stage of spermatogenesis, our genetic evidence underscores that RNF8 is not involved in histone-to-protamine exchange.

Indexed as

SpermatogenesisAcetylationAnimalsBiological TransportChromatin Assembly and DisassemblyHistonesMiceMice, KnockoutProtaminesSex ChromosomesUbiquitinationUbiquitin-Protein LigasesHistonesProtaminesRnf8 protein, mouseUbiquitin-Protein Ligaseshistone-to-protamine exchangeMIWIRNF8spermspermiogenesis

Identifiers

PMID34225362
PMCPMC8599036
OpenAlexW3179735841

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.