ArticleProceedings of the National Academy of Sciences of the United States of America1988
Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Maternal corticotropin-releasing hormone is associated with LEP DNA methylation at birth and in childhood: an epigenome-wide study in Project Viva.International journal of obesity (2005) · 2019Article
- Gene expression profiling during the embryo-to-larva transition in the giant red sea urchinEcology and evolution · 2017Article
- Glucose-6-phosphate dehydrogenase, NADPH, and cell survival.IUBMB life · 2012Review
- Fluorogenic metabolic probes for direct activity readout of redox enzymes: Selective measurement of human AKR1C2 in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The dynamics of local kinetic parameters of glutamate dehydrogenase in rat liver.Histochemistry and cell biology · 1996Article
- Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver.Clinical & experimental metastasis · 1996Article
- Heterogeneity of kinetic parameters of enzymes in situ in rat liver lobules.Histochemistry and cell biology · 1995Review
- Analysis of enzyme reactions in situ.The Histochemical journal · 1995Review
- Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts.Biophysical journal · 1994Article
- Isolation and the complete amino acid sequence of lumenal endoplasmic reticulum glucose-6-phosphate dehydrogenase.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Phosphoprotein inhibition of calcium-stimulated exocytosis in sea urchin eggs.The Journal of cell biology · 1991Article
- Guanosine 5'-thiotriphosphate may stimulate phosphoinositide messenger production in sea urchin eggs by a different route than the fertilizing sperm.Cell regulation · 1991Article
- Stable, resealable pores formed in sea urchin eggs by electric discharge (electroporation) permit substrate loading for assay of enzymes in vivo.Cell regulation · 1989Article
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Authors and funding
2 authors.
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Abstract
Sea urchin eggs and embryos subjected to high-voltage electric discharge in a medium mimicking the intracellular milieu retain their structural integrity and remain permeable, permitting substrates to enter the cytoplasm and thus assay of enzyme activity. At saturating concentrations of substrates, five of six enzymes assayed for more active (three to fifteen times) in permeabilized embryos than in permeabilized eggs, but no fertilization-related differences are seen in homogenates prepared from these same permeabilized cells. Furthermore, enzyme activity in homogenates always exceeds that in the permeabilized cell suspensions. This difference in enzyme reaction rates between unfertilized eggs and fertilized eggs is not due to differences in the diffusibility of substrates into the permeabilized cells. The activity of glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP+ 1-oxidoreductase, EC 1.1.1.49) in permeabilized cells was studied in greater detail and has the following characteristics. (i) Regulation of activity persists during early development. (ii) This regulation is not mediated by diffusible allosteric agents. (iii) Stimulation at fertilization is initiated by a rise in intracellular calcium and is further promoted by cytoplasmic alkalinization. (iv) The microenvironment experienced by this enzyme intracellularly differs from that of the enzyme in homogenates as evidenced by markedly different pH vs. activity profiles. These results indicate that the regulatory status of enzymes is preserved in electrically permeabilized cells and suggest that this regulation depends on some cell structural feature(s) that is (are) destroyed upon homogenization.
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