Evidence map›Paper›PMID 34224206›Full record

ArticleChemMedChem2021

A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.

David K Heidary, Sarah M Kriger, Austin C Hachey, Edith C Glazer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ChemMedChem, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Design of Cytochrome P450 1B1 InhibitorsJournal of medicinal chemistry · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David K HeidaryDepartment of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506, USA.
Sarah M KrigerDepartment of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506, USA.
Austin C HacheyDepartment of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506, USA.
Edith C GlazerDepartment of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506, USA.ORCID 0000-0002-0190-7742

Funding

Role of small molecule interactions and multiprotein assemblies in CYP1B1 disease-associated function and dysfunctionR01GM138882 · NIGMS · UNIVERSITY OF KENTUCKY · PI GLAZER, EDITH C, HEIDARY, DAVID · 2020 to 2024
$1.9M
National Institute of General Medical Sciences of the US National Institutes of Health R01GM138882NIGMS NIH HHS R01 GM138882University of Kentucky Light Microscopy Core
6 · The paper itself

Abstract

Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates.

Indexed as

FluorometryAromataseAromatase InhibitorsDose-Response Relationship, DrugFluorescent DyesHEK293 CellsHumansMolecular StructureStructure-Activity RelationshipAromataseAromatase InhibitorsCYP19A1 protein, humanFluorescent DyesAromataseAssay developmentBreast cancerCYP19A1Cytochrome P450

Identifiers

PMID34224206
PMCPMC9276262

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.