Evidence map›Paper›PMID 34220409›Full record

ArticleAdvanced functional materials2020

Coupling Nanostructured Microchips with Covalent Chemistry Enables Purification of Sarcoma-Derived Extracellular Vesicles for Downstream Functional Studies.

Jiantong Dong, Ryan Y Zhang, Na Sun, Junhui Hu, Matthew D Smalley, Anqi Zhou, Hua Yue, Winston Rothermich, Mengxiang Chen, Jiayuan Chen and 13 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced functional materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.

  1. Pooled it
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  12. B7-H3-liquid biopsy for the characterization and monitoring of the dynamic biology of prostate cancer.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 6 institutions in 2 countries.

Jiantong DongCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Ryan Y ZhangCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Na SunCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Junhui HuDepartment of Molecular and Medical Pharmacology David Geffen School of Medicine UCLA 650 Charles E Young Dr., Los Angeles, CA 90095, USA.
Matthew D SmalleyCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Anqi ZhouCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Hua YueCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Winston RothermichCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Mengxiang ChenCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Jiayuan ChenCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Jinglei YeCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Pai-Chi TengCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Dongping QiCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Jeffrey A ToretskyDepartments of Oncology and Pediatrics Georgetown University 3970 Reservoir Rd NW, Washington, DC 20057, USA.
James S TomlinsonDepartment of Surgery UCLA 200 Medical Plaza, Los Angeles, CA 90024, USA.
Mengyuan LiBeijing National Laboratory for Molecular Sciences MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering College of Chemistry and Molecular Engineering Peking University 202 Chengfu Road, Haidian District, Beijing 100871, China.
Paul S WeissCalifornia NanoSystems Institute Departments of Chemistry and Biochemistry Bioengineering, and Materials Science and Engineering UCLA 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Steven J JonasDepartment of Pediatrics Ronald Reagan UCLA Medical Center UCLA Mattel Children's Hospital 10833 Le Conte Ave, Los Angeles, CA 90095, USA.
Noah FedermanDepartment of Pediatrics Ronald Reagan UCLA Medical Center UCLA Mattel Children's Hospital 10833 Le Conte Ave, Los Angeles, CA 90095, USA.
Lily WuDepartment of Molecular and Medical Pharmacology David Geffen School of Medicine UCLA 650 Charles E Young Dr., Los Angeles, CA 90095, USA.
Meiping ZhaoBeijing National Laboratory for Molecular Sciences MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering College of Chemistry and Molecular Engineering Peking University 202 Chengfu Road, Haidian District, Beijing 100871, China.
Hsian-Rong TsengCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Yazhen ZhuCalifornia NanoSystems Institute Crump Institute for Molecular Imaging Department of Molecular and Medical Pharmacology University of California Los Angeles (UCLA) 570 Westwood Plaza, Los Angeles, CA 90095, USA.
California NanoSystems Institute · USPeking University · CNRonald Reagan UCLA Medical Center · USUniversity of California, Los Angeles · USGeorgetown University · USUCLA Medical Center · US

Funding

Non-Invasive Prenatal Diagnostics Based on Circulating TrophoblastsU01EB026421 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PISARSKA, MARGARETA, TSENG, HSIAN-RONG · 2019 to 2023
$4.0M
HCC EV Digital Scoring Assay for assessing treatment response in HCC patientsR01CA253651 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2024
$3.3M
Extracellular Vesicle-Based Digital Scoring Assay for Detecting Early-stage Hepatocellular CarcinomaR01CA255727 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHU, YAZHEN · 2021 to 2025
$3.2M
A blood test to identify prostate cancer patients at risk for visceral metastasisR01CA218356 · NCI · CEDARS-SINAI MEDICAL CENTER · PI POSADAS, EDWIN MELENCIO, TSENG, HSIAN-RONG · 2017 to 2021
$3.0M
Integrated analysis of HCC CTCs for Liver Transplant Candidate SelectionR01CA246304 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2024
$2.8M
Thermoresponsive NanoVelcro CTC Purification System for Prostate Cancer ProfilingU01CA198900 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI POSADAS, EDWIN MELENCIO, TSENG, HSIAN-RONG · 2015 to 2019
$2.4M
Click Chemistry-Mediated Microfluidic Sorting for HCC CTCsR21CA235340 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2019 to 2021
$543k
Biomimetic NanoVilli Chips for Extracellular Vesicles in Hepatocellular CarcinomaR21CA240887 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2021
$419k
NCI NIH HHS R01 CA218356NCI NIH HHS R01 CA246304NCI NIH HHS R01 CA253651NCI NIH HHS R01 CA255727NCI NIH HHS R21 CA235340NCI NIH HHS R21 CA240887NCI NIH HHS U01 CA198900NIBIB NIH HHS U01 EB026421
6 · The paper itself

Abstract

Tumor-derived extracellular vesicles (EVs) play essential roles in intercellular communication during tumor growth and metastatic evolution. Currently, little is known about the possible roles of tumor-derived EVs in sarcoma because the lack of specific surface markers makes it technically challenging to purify sarcoma-derived EVs. In this study, a specific purification system is developed for Ewing sarcoma (ES)-derived EVs by coupling covalent chemistry-mediated EV capture/ release within a nanostructure-embedded microchip. The purification platform-ES-EV Click Chip-takes advantage of specific anti-LINGO-1 recognition and sensitive click chemistry-mediated EV capture, followed by disulfide cleavage-driven EV release. Since the device is capable of specific and efficient purification of intact ES EVs with high purity, ES-EV Click Chip is ideal for conducting downstream functional studies of ES EVs. Absolute quantification of the molecular hallmark of ES (i.e., EWS rearrangements) using reverse transcription Droplet Digital PCR enables specific quantification of ES EVs. The purified ES EVs can be internalized by recipient cells and transfer their mRNA cargoes, exhibiting their biological intactness and potential role as biological shuttles in intercellular communication.

Indexed as

covalent chemistryextracellular vesiclesmicrofluidicsnanostructured substratessarcoma

Identifiers

PMID34220409
PMCPMC8248519
OpenAlexW3087392579

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.