ArticleScientific reports2021
Interlaboratory study for the evaluation of three microtiter plate-based biofilm quantification methods.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review of the use of bacteriophages for in vitro biofilm control.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Pooled it
- Dimensional analysis as a tool for biofilm comparative analysis: bridging the interdisciplinary divides.Journal of bacteriology · 2026Review
- A Lytic Bacteriophage Cocktail Attenuates Carbapenem-Resistant Klebsiella pneumoniae-Acinetobacter baumannii Mixed-Species Biofilms and Reshapes Transcriptional Responses.Microbial biotechnology · 2026Article
- Characterization of a Novel Endolysin Sb1-LysAEJ as a Biocide and Food-contact Surface Disinfectant for the Control of Staphylococcus aureus.Probiotics and antimicrobial proteins · 2026Article
- Phenotypic and Genomic Analysis of Swine-DerivedAnimals : an open access journal from MDPI · 2026Article
- Biofilm sensitization to breakJournal of medical microbiology · 2026Article
- Biofilm formation and antimicrobial resistance of Pseudomonas aeruginosa in cheese production systems.Scientific reports · 2026Article
- Can commensals alter pathogen's antibiotic resistance during co-culture?Journal of medical microbiology · 2026Article
- Copper biosorption by Serratia plymuthica: crucial role of tightly bound extracellular polymeric substances in planktonic and biofilm systems.Biodegradation · 2026Article
- Establishing a quantitative link between crystal violet absorbance and biomass in biofilms.MethodsX · 2025Article
- Machine Learning-Identified Potent Antimicrobial Peptides Against Multidrug-Resistant Bacteria and Skin Infections.Antibiotics (Basel, Switzerland) · 2025Article
- Widespread Distribution of Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Is Increased Biofilm Formation Associated with Decreased Antimicrobial Susceptibility? A Systematic Literature Review.Microorganisms · 2025Review
- Detection of Bacterial Activity via Reduction of Prussian Blue Thin Films as Probed by Raman Spectroscopy.ACS omega · 2025Article
- A guide to publishing inBiofilm · 2025Article
- In-vitro and in-silico antibacterial and antibiofilm activities of an aromatic heterocyclic metabolite from a novel halo-thermophilic Streptomyces sp. strain CBN-1 against bacteria causing nosocomial infections.Molecular biology reports · 2025Article
- Identification and Antimicrobial Potential of Marine Sponges (International journal of microbiology · 2025Article
- Multifaceted antimicrobial mechanisms of NCR147-derived peptides fromFrontiers in microbiology · 2025Article
- Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy.Biofilm · 2024Article
- Lentisk (Pharmaceutics · 2024Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these methods have often been difficult to reproduce. Hence, it is important to obtain a better understanding of the repeatability and reproducibility of these methods. An interlaboratory study was performed in five different laboratories to evaluate the reproducibility and responsiveness of three methods to quantify Staphylococcus aureus biofilm formation in 96-well microtiter plates: crystal violet, resazurin, and plate counts. An inter-lab protocol was developed for the study. The protocol was separated into three steps: biofilm growth, biofilm challenge, biofilm assessment. For control experiments participants performed the growth and assessment steps only. For treatment experiments, all three steps were performed and the efficacy of sodium hypochlorite (NaOCl) in killing S. aureus biofilms was evaluated. In control experiments, on the log
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.