Evidence map›Paper›PMID 34215581›Full record

ArticleScience advances2021

Synthetic auxotrophy remains stable after continuous evolution and in coculture with mammalian cells.

Aditya M Kunjapur, Michael G Napolitano, Eriona Hysolli, Karen Noguera, Evan M Appleton, Max G Schubert, Michaela A Jones, Siddharth Iyer, Daniel J Mandell, George M Church

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Article
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  10. In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins.Chembiochem : a European journal of chemical biology · 2025
    Review
  11. Review
  12. Review
  13. Article
  14. Review
  15. Review
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  17. Article
  18. Engineering bacterial theranostics: from logic gates toFrontiers in bioengineering and biotechnology · 2024
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Aditya M KunjapurDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA. kunjapur@udel.edu gchurch@genetics.med.harvard.edu.ORCID http://orcid.org/0000-0001-6869-9530
Michael G NapolitanoDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.ORCID http://orcid.org/0000-0002-2269-5603
Eriona HysolliDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.ORCID http://orcid.org/0000-0002-9677-7169
Karen NogueraDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.ORCID http://orcid.org/0000-0002-5037-3863
Evan M AppletonDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.ORCID http://orcid.org/0000-0002-9378-5913
Max G SchubertDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.
Michaela A JonesDepartment of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy Street, CLB 215, Newark, DE 19716, USA.
Siddharth IyerDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.ORCID http://orcid.org/0000-0003-4986-4275
Daniel J MandellDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA.
George M ChurchDepartment of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 238, Boston, MA 02115, USA. kunjapur@udel.edu gchurch@genetics.med.harvard.edu.ORCID http://orcid.org/0000-0002-0775-2913

Funding

Chemistry-Biology Interface Predoctoral Training Grant 2024-2029T32GM133395 · NIGMS · UNIVERSITY OF DELAWARE · PI Catherine Leimkuhler Grimes · 2019 to 2026
$3.7M
NIGMS NIH HHS T32 GM133395
6 · The paper itself

Abstract

Understanding the evolutionary stability and possible context dependence of biological containment techniques is critical as engineered microbes are increasingly under consideration for applications beyond biomanufacturing. While synthetic auxotrophy previously prevented

Identifiers

PMID34215581
PMCPMC11060021

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.