Evidence map›Paper›PMID 34212160›Full record

ArticleAdvanced nanobiomed research2021

Labeling of Erythrocytes by Porphyrin-Phospholipid.

Sunanda Kumar, Dawei Jiang, Boyang Sun, Kaelyn V Seeley, Jonathan W Engle, Zachary Sia, Xuedan He, Sriram Neelamegham, Weibo Cai, Jonathan F Lovell

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced nanobiomed research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Homotypic Targeting of [Pharmaceutics · 2025
    Article
  3. Direct Cell Radiolabeling forChemical reviews · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Sunanda KumarDepartment of Biomedical Engineering, State University of New York at Buffalo, NY 14260, USA.
Dawei JiangDepartment of Materials Science and Engineering, Department of Radiology, Department of Medical Physics, and University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Madison, Wisconsin 53705, United States.
Boyang SunDepartment of Biomedical Engineering, State University of New York at Buffalo, NY 14260, USA.
Kaelyn V SeeleyDepartment of Materials Science and Engineering, Department of Radiology, Department of Medical Physics, and University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Madison, Wisconsin 53705, United States.
Jonathan W EngleDepartment of Materials Science and Engineering, Department of Radiology, Department of Medical Physics, and University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Madison, Wisconsin 53705, United States.
Zachary SiaDepartment of Biomedical Engineering, State University of New York at Buffalo, NY 14260, USA.
Xuedan HeDepartment of Biomedical Engineering, State University of New York at Buffalo, NY 14260, USA.
Sriram NeelameghamDepartment of Chemical Engineering, State University of New York at Buffalo, NY 14260, USA.
Weibo CaiDepartment of Materials Science and Engineering, Department of Radiology, Department of Medical Physics, and University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, Madison, Wisconsin 53705, United States.
Jonathan F LovellDepartment of Biomedical Engineering, State University of New York at Buffalo, NY 14260, USA.
University at Buffalo, State University of New York · USUniversity of Wisconsin–Madison · US

Funding

Light-Triggered Drug Release in Primed Pancreatic TumorsR01EB017270 · NIBIB · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LOVELL, JONATHAN F · 2013 to 2021
$3.5M
NIBIB NIH HHS R01 EB017270
6 · The paper itself

Abstract

A method is developed for membrane labeling of erythrocytes with porphyrin-phospholipid (PoP). To generate a concentrated PoP solution for labeling human red blood cells (RBCs), various surfactants and solvents are screened to identify conditions that avoid hemolysis, while minimizing non-specific PoP co-precipitation with RBCs in the pellet during centrifugation washes. Cholate, Tween 80 and Tween 40 are identified as useful surfactants for this purpose. When labeled RBCs are mixed with unlabeled ones, substantial non-specific PoP exchange is observed. Egg-yolk lecithin is included in a washing buffer to remove loosely bound PoP and reduce PoP exchange with unlabeled erythrocytes, based on flow cytometry and photodynamic hemolysis assays. Murine RBCs that are labeled with

Indexed as

erythrocytelabelinglipidsmembraneporphyrin

Identifiers

PMID34212160
PMCPMC8240482
OpenAlexW3095880691

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.