ArticleAdvanced functional materials2020
Metabolic Labeling to Probe the Spatiotemporal Accumulation of Matrix at the Chondrocyte-Hydrogel Interface.
Article in Advanced functional materials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 82 citations in OpenAlex.
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- Engineering Next-generation Drug Delivery Systems Via 4-D Printing: A Paradigm Shift Toward Precision Medicine.AAPS PharmSciTech · 2026Review
- Tracking Spatiotemporal Extracellular Matrix Evolution and Tissue Fusion in 3D Microtissues via Click Chemistry-Based Metabolic Labelling.Advanced healthcare materials · 2026Article
- Nascent extracellular matrix: the missing piece in hydrogel design.Cell biomaterials · 2026Article
- Tuning scaffold degradation with non-natural peptidomimetics to control human umbilical vein endothelial cell morphology and vessel formation.Acta biomaterialia · 2026Article
- Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.Annals of biomedical engineering · 2026Article
- Fiber-Reinforced Composites for Vaginal Tissue Engineering Applications.Journal of biomedical materials research. Part A · 2026Article
- Mechanical Cues Regulate Cargo Sorting and Export at the Golgi.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Rapid restoration of cell phenotype and matrix forming capacity following transient nuclear softening.Acta biomaterialia · 2025Article
- Decorin Promotes Nascent Proteoglycan Retention in Cartilage Matrix by Strengthening Collagen II-Aggrecan Integration.bioRxiv : the preprint server for biology · 2025Article
- Effects of Stiffness and Degradability on Cardiac Fibroblast Contractility and Extracellular Matrix Secretion in Three-Dimensional Hydrogel Scaffolds.ACS biomaterials science & engineering · 2025Article
- Hydrogel Viscoelasticity Modulates Cell Nascent Extracellular Matrix Deposition.Macromolecular rapid communications · 2025Article
- Tuning Scaffold Degradation with Non-Natural Peptidomimetics to Control Human Umbilical Vein Endothelial Cell Morphology and Vessel Formation.bioRxiv : the preprint server for biology · 2025Article
- Engineered basement membrane mimetic hydrogels to study mammary epithelial morphogenesis and invasion.Science advances · 2025Article
- 4D-printed microdevices for spatiotemporal detection of ctDNA and miRNA in pancreatic cancer: an in-depth review.Medical oncology (Northwood, London, England) · 2025Review
- Real-time monitoring of cell membrane viscosity in a 3D hydrogel cultivation microenvironment using BODIPY-based fluorescent probe.Analytical and bioanalytical chemistry · 2025Article
- Tuning local matrix compliance accelerates mesenchymal stem cell chondrogenesis in 3D sliding hydrogels.Biomaterials · 2025Article
- 3D bioprinted scaffolds for osteochondral regeneration: advancements and applications.Materials today. Bio · 2025Review
- Sex-stratified osteochondral organ-on-chip model reveals sex-specific responses to inflammatory stimulation.Materials today. Bio · 2025Article
- Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids.Stem cell reports · 2025Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Hydrogels are engineered with biochemical and biophysical signals to recreate aspects of the native microenvironment and to control cellular functions such as differentiation and matrix deposition. This deposited matrix accumulates within the pericellular space and likely affects the interactions between encapsulated cells and the engineered hydrogel; however, there has been little work to study the spatiotemporal evolution of matrix at this interface. To address this, metabolic labeling is employed to visualize the temporal and spatial positioning of nascent proteins and proteoglycans deposited by chondrocytes. Within covalently crosslinked hyaluronic acid hydrogels, chondrocytes deposit nascent proteins and proteoglycans in the pericellular space within 1 d after encapsulation. The accumulation of this matrix, as measured by an increase in matrix thickness during culture, depends on the initial hydrogel crosslink density with decreased thicknesses for more crosslinked hydrogels. Encapsulated fluorescent beads are used to monitor the hydrogel location and indicate that the emerging nascent matrix physically displaces the hydrogel from the cell membrane with extended culture. These findings suggest that secreted matrix increasingly masks the presentation of engineered hydrogel cues and may have implications for the design of hydrogels in tissue engineering and regenerative medicine.
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Registered trials
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