ArticleScientific reports2021
Local application of Usag-1 siRNA can promote tooth regeneration in Runx2-deficient mice.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- USAG-1/SOSTDC1 as a Candidate Target for Periodontal Regeneration: Biological Rationale, Knowledge Gaps, and Future Directions.Molecules (Basel, Switzerland) · 2026Review
- Stem Cell-based Therapeutic and Endogenous Regeneration Approaches for Congenital Tooth Agenesis.Stem cell reviews and reports · 2026Review
- Stem cell-driven biomedical technologies for tooth regeneration: engineering scaffolds, organoid models, and molecular targeted strategies.Stem cell research & therapy · 2026Review
- Resilience of the replacing dentition in adult reptiles.Developmental biology · 2024Review
- Advances in tooth agenesis and tooth regeneration.Regenerative therapy · 2023Review
- Tooth number abnormality: from bench to bedside.International journal of oral science · 2023Review
- Article
- Irisin improves delayed bone repair in diabetic female mice.Journal of bone and mineral metabolism · 2022Article
- Exosome mediated biological functions within skeletal microenvironment.Frontiers in bioengineering and biotechnology · 2022Review
- Supernumerary teeth: A pictorial review and revised classification.Journal of oral biology and craniofacial researchReview
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Runt-related transcription factor 2 (Runx2)-deficient mice can be used to model congenital tooth agenesis in humans. Conversely, uterine sensitization-associated gene-1 (Usag-1)-deficient mice exhibit supernumerary tooth formation. Arrested tooth formation can be restored by crossing both knockout-mouse strains; however, it remains unclear whether topical inhibition of Usag-1 expression can enable the recovery of tooth formation in Runx2-deficient mice. Here, we tested whether inhibiting the topical expression of Usag-1 can reverse arrested tooth formation after Runx2 abrogation. The results showed that local application of Usag-1 Stealth small interfering RNA (siRNA) promoted tooth development following Runx2 siRNA-induced agenesis. Additionally, renal capsule transplantation of siRNA-loaded cationized, gelatin-treated mouse mandibles confirmed that cationized gelatin can serve as an effective drug-delivery system. We then performed renal capsule transplantation of wild-type and Runx2-knockout (KO) mouse mandibles, treated with Usag-1 siRNA, revealing that hindered tooth formation was rescued by Usag-1 knockdown. Furthermore, topically applied Usag-1 siRNA partially rescued arrested tooth development in Runx2-KO mice, demonstrating its potential for regenerating teeth in Runx2-deficient mice. Our findings have implications for developing topical treatments for congenital tooth agenesis.
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