Evidence map›Paper›PMID 34211055›Full record

ArticleScientific reports2021

A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line.

D C Soler, T Kowatz, A E Sloan, T S McCormick, K D Cooper, R Stepanyan, A Engel, A Vahedi-Faridi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Classification and Gene Structure of Aquaporins.Advances in experimental medicine and biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

D C SolerThe Department of Neurosurgery, Case Western Reserve University, Cleveland, USA. david.soler@case.edu.
T KowatzDepartment of Physiology and Biophysics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106-4965, USA.
A E SloanThe Department of Neurosurgery, Case Western Reserve University, Cleveland, USA.
T S McCormickDepartment of Dermatology, Case Western Reserve University, Cleveland, USA.
K D CooperDepartment of Dermatology, Case Western Reserve University, Cleveland, USA.
R StepanyanDepartment of Otolaryngology-HNS, Case Western Reserve University, Cleveland, USA.
A EngelBiozentrum, University of Basel, Basel, Switzerland.
A Vahedi-FaridiDepartment of Physiology and Biophysics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106-4965, USA.
Case Western Reserve University · USUniversity Hospitals of Cleveland · USUniversity of Basel · CH

Funding

Mechanotransduction and calcium regulation in cochlear hair cellsR01DC015016 · NIDCD · CASE WESTERN RESERVE UNIVERSITY · PI STEPANYAN, RUBEN · 2016 to 2020
$1.7M
Leica SP8 Confocal with Hyvolution2 DeconvolutionS10OD024996 · OD · CASE WESTERN RESERVE UNIVERSITY · PI IMANISHI, YOSHIKAZU · 2018 to 2018
$594k
NIDCD NIH HHS R01 DC015016NIH HHS S10 OD024996
6 · The paper itself

Abstract

The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6

Indexed as

Amino Acid SequenceAnimalsAquaporin 6Cell MembraneHEK293 CellsHumansProtein ConformationProtein TransportRatsAquaporin 6

Identifiers

PMID34211055
PMCPMC8249660
OpenAlexW3174603103

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.