ArticleNPJ biofilms and microbiomes2021
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri.
Article in NPJ biofilms and microbiomes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Regulatory mechanisms of nitrogen homeostasis in insect growth and development.Insect science · 2026Review
- A bacterial ally for nitrogen-fixing biofilm: enhancing the rhizosphere colonization ofApplied and environmental microbiology · 2026Article
- Mechanisms of Pseudomonas spp. as plant growth promoting rhizobacteria: plant nutrition and biocontrol.Antonie van Leeuwenhoek · 2026Review
- Enhanced anti Pseudomonas aeruginosa efficacy of niosomal-encapsulated Frondoside A from sea cucumber: a novel biotechnological approach.BMC biotechnology · 2026Article
- An engineered Pseudomonas stutzeri strain overexpressing the sigma factor AlgU promotes maize growth under salt stress.Applied microbiology and biotechnology · 2026Article
- UnderstandingMicroorganisms · 2026Review
- RpoN mediates biofilm formation by directly controllingBiofilm · 2025Article
- Genetic basis of biofilm formation and salt adaptation in the plant-beneficial strain Stutzerimonas stutzeri MJL19.Applied microbiology and biotechnology · 2025Article
- Virulence-linked adhesin drives mutualist colonization of the bee gut via biofilm formation.bioRxiv : the preprint server for biology · 2024Article
- Article
- Establishment of a Human Immunocompetent 3D Tissue Model to Enable the Long-Term Examination of Biofilm-Tissue Interactions.Bioengineering (Basel, Switzerland) · 2024Article
- Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.ACS synthetic biology · 2023Article
- Article
- Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes iniScience · 2022Article
- Studying Plant-Insect Interactions through the Analyses of the Diversity, Composition, and Functional Inference of Their Bacteriomes.Microorganisms · 2022Article
- RpoN is required for the motility and contributes to the killing ability of Plesiomonas shigelloides.BMC microbiology · 2022Article
- Post-transcriptional control of bacterial nitrogen metabolism by regulatory noncoding RNAs.World journal of microbiology & biotechnology · 2022Review
- Article
- The Regulatory Functions of σInternational journal of molecular sciences · 2021Review
- CdgC, a Cyclic-di-GMP Diguanylate Cyclase ofFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm and nitrogen fixation are two competitive strategies used by many plant-associated bacteria; however, the mechanisms underlying the formation of nitrogen-fixing biofilms remain largely unknown. Here, we examined the roles of multiple signalling systems in the regulation of biofilm formation by root-associated diazotrophic P. stutzeri A1501. Physiological analysis, construction of mutant strains and microscale thermophoresis experiments showed that RpoN is a regulatory hub coupling nitrogen fixation and biofilm formation by directly activating the transcription of pslA, a major gene involved in the synthesis of the Psl exopolysaccharide component of the biofilm matrix and nifA, the transcriptional activator of nif gene expression. Genetic complementation studies and determination of the copy number of transcripts by droplet digital PCR confirmed that the regulatory ncRNA RsmZ serves as a signal amplifier to trigger biofilm formation by sequestering the translational repressor protein RsmA away from pslA and sadC mRNAs, the latter of which encodes a diguanylate cyclase that synthesises c-di-GMP. Moreover, RpoS exerts a braking effect on biofilm formation by transcriptionally downregulating RsmZ expression, while RpoS expression is repressed posttranscriptionally by RsmA. These findings provide mechanistic insights into how the Rpo/Gac/Rsm regulatory networks fine-tune nitrogen-fixing biofilm formation in response to the availability of nutrients.
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