Evidence map›Paper›PMID 34210798›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

A fluorescence-based genetic screen reveals diverse mechanisms silencing small RNA signaling in

Jiandong Chen, Leann To, Francois de Mets, Xing Luo, Nadim Majdalani, Chin-Hsien Tai, Susan Gottesman

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Structural and biophysical insights into the unique RNase YicC.bioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. What makes a small RNA work?Nucleic acids research · 2025
    Review
  6. Review
  7. Tevenvirinae phages encode a family of LSm-like fold proteins.Computational and structural biotechnology journal · 2025
    Article
  8. Divergent Molecular Responses to Heavy Water inPlants (Basel, Switzerland) · 2024
    Article
  9. Article
  10. Review
  11. RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024
    Review
  12. Directed Screening for sRNA Targets in E. coli Using a Plasmid Library.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  13. Article
  14. Article
  15. An acetyltranferase moonlights as a regulator of the RNA binding repertoire of the RNA chaperone Hfq inProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  16. Article
  17. Role of themBio · 2023
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jiandong ChenLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892 gottesms@nih.gov jiandong.chen@pennmedicine.upenn.edu.
Leann ToLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Francois de MetsLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Xing LuoLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.ORCID 0000-0002-1137-0571
Nadim MajdalaniLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.ORCID 0000-0002-9314-5203
Chin-Hsien TaiLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
Susan GottesmanLaboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892 gottesms@nih.gov jiandong.chen@pennmedicine.upenn.edu.ORCID 0000-0002-7792-1284

Funding

Bacterial Functions Involved in Cell Growth ControlZIABC008714 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI GOTTESMAN, SUSAN · 2009 to 2025
$20.3M
6 · The paper itself

Abstract

As key players of gene regulation in many bacteria, small regulatory RNAs (sRNAs) associated with the RNA chaperone Hfq shape numerous phenotypic traits, including metabolism, stress response and adaptation, as well as virulence. sRNAs can alter target messenger RNA (mRNA) translation and stability via base pairing. sRNA synthesis is generally under tight transcriptional regulation, but other levels of regulation of sRNA signaling are less well understood. Here we used a fluorescence-based functional screen to identify regulators that can quench sRNA signaling of the iron-responsive sRNA RyhB in

Indexed as

Gene SilencingGenetic TestingSignal TransductionAcetyltransferasesChromosomes, BacterialEscherichia coliEscherichia coli ProteinsFluorescenceGene Expression Regulation, BacterialGenes, ReporterGenome, BacterialPlasmidsProteolysisRecombinant Fusion ProteinsRibonucleasesRNA, BacterialAcetyltransferasesEscherichia coli ProteinsRecombinant Fusion ProteinsRibonucleasesRNA, BacterialexoribonucleaseHfq competitionRNA chaperoneRNA spongeRyhB

Identifiers

PMID34210798
PMCPMC8271630

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.