Investigation of the Detailed AMPylated Reaction Mechanism for the Huntingtin Yeast-Interacting Protein E Enzyme HYPE.

Meili Liu, Zhe Huai, Hongwei Tan, Guangju Chen

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In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

0.4field-weighted citation impact, top 35% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

FICD (FIC Domain Protein Adenylyl Transferase) Deficiency Protects Mice From Hypertrophy-Induced Heart Failure and Promotes BiP (Binding Immunoglobulin Protein) -Mediated Activation of the Unfolded Protein Response and Endoplasmic Reticulum-Selective Autophagy in Cardiomyocytes.Journal of the American Heart Association · 2025
Article
Catalytic deAMPylation in AMPylation-inhibitory/assistant forms of FICD protein.Frontiers in chemistry · 2023
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Meili LiuDepartment of Chemistry, Beijing Normal University, Beijing 100875, China.

Zhe HuaiState Key Laboratory of Precision Spectroscopy, School of Physics and Electronic Science, East China Normal University, Shanghai 200062, China.ORCID 0000-0002-6759-5104

Hongwei TanDepartment of Chemistry, Beijing Normal University, Beijing 100875, China.ORCID 0000-0002-1376-6019

Guangju ChenDepartment of Chemistry, Beijing Normal University, Beijing 100875, China.

Beijing Normal University · CNEast China Normal University · CN

Funding

National Natural Science Foundation of China,the National Key Research and Development Program of China no. 22073010, 21571019 and 21573020,no. 2019YFC1805600

6 · The paper itself

Abstract

AMPylation is a prevalent posttranslational modification that involves the addition of adenosine monophosphate (AMP) to proteins. Exactly how Huntingtin-associated yeast-interacting protein E (HYPE), as the first human protein, is involved in the transformation of the AMP moiety to its substrate target protein (the endoplasmic reticulum chaperone binding to immunoglobulin protein (BiP)) is still an open question. Additionally, a conserved glutamine plays a vital key role in the AMPylation reaction in most filamentation processes induced by the cAMP (Fic) protein. In the present work, the detailed catalytic AMPylation mechanisms in HYPE were determined based on the density functional theory (DFT) method. Molecular dynamics (MD) simulations were further used to investigate the exact role of the inhibitory glutamate. The metal center, Mg

Indexed as

Protein Processing, Post-TranslationalAdenosine MonophosphateCrystallography, X-RayEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsHumansMembrane ProteinsMetabolic Networks and PathwaysModels, MolecularMolecular Dynamics SimulationNucleotidyltransferasesProtein BindingProtein Interaction Domains and MotifsProtein Interaction MappingAdenosine MonophosphateEndoplasmic Reticulum Chaperone BiPFICD protein, humanHeat-Shock ProteinsMembrane ProteinsNucleotidyltransferasesAMPylationdensity functional theoryFic-mediatedHYPE

Identifiers

PMID34209803

DOI10.3390/ijms22136999

PMCPMC8267892

OpenAlexW3173884185

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LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.