ArticleAnimals : an open access journal from MDPI2021
Heat Shock Protein 70 Improves In Vitro Embryo Yield and Quality from Heat Stressed Bovine Oocytes.
Article in Animals : an open access journal from MDPI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Saliva-based RT-LAMP assays support heat shock protein 70 as a promising transcript marker for estrus identification in buffaloes.Tropical animal health and production · 2026Article
- Establishment and characterization of Hanwoo cumulus cell line for heat stress studies.Animal bioscience · 2026Article
- Dose-Dependent Effects of Heat Shock Cognate 70 on Viability and Apoptosis-Related Gene Expression in In Vitro-Produced Bovine Embryos.Veterinary sciences · 2026Article
- The addition of heat shock protein 70 toOpen veterinary journal · 2025Article
- Post-warming quality of goat oocytes under heat shock stress: A study of the maturation rate, heat shock protein-70, adenosine triphosphate, and glutathione levels.Veterinary world · 2025Article
- The Importance of Mitochondrial Processes in the Maturation and Acquisition of Competences of Oocytes and Embryo Culture.International journal of molecular sciences · 2025Review
- The role of heat shock protein 70 on oocyte apoptosis during vitrification.Open veterinary journal · 2025Review
- Impact of Heat Stress on Oocyte Developmental Competence and Pre-Implantation Embryo Viability in Cattle.Animals : an open access journal from MDPI · 2024Review
- Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis.Antioxidants (Basel, Switzerland) · 2024Review
- Use of platelet-rich plasma on in vitro maturation during bovine embryo production.Animal reproduction · 2024Article
- Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization.International journal of molecular sciences · 2023Article
- Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows.Animals : an open access journal from MDPI · 2023Review
- Effects of Heat Stress in Dairy Cows Raised in the Confined System: A Scientometric Review.Animals : an open access journal from MDPI · 2023Review
- Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study.Journal of animal science and biotechnology · 2022Article
- The Effects of Heat Shock Protein 70 Addition in the Culture Medium on the Development and Quality of In Vitro Produced Heat Shocked Bovine Embryos.Animals : an open access journal from MDPI · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock protein 70 (HSP70) is a chaperon that stabilizes unfolded or partially folded proteins, preventing inappropriate inter- and intramolecular interactions. Here, we examined the developmental competence of in vitro matured oocytes exposed to heat stress with or without HSP70. Bovine oocytes were matured for 24 h at 39 °C without (group C39) or with HSP70 (group H39) and at 41 °C for the first 6 h, followed by 16 h at 39 °C with (group H41) or without HSP70 (group C41). After insemination, zygotes were cultured for 9 days at 39 °C. Cleavage and embryo yield were assessed 48 h post insemination and on days 7, 8, 9, respectively. Gene expression was assessed by RT-PCR in oocytes, cumulus cells and blastocysts. In C41, blastocysts formation rate was lower than in C39 and on day 9 it was lower than in H41. In oocytes, HSP70 enhanced the expression of three HSP genes regardless of incubation temperature. HSP70 at 39 °C led to tight coordination of gene expression in oocytes and blastocysts, but not in cumulus cells. Our results imply that HSP70, by preventing apoptosis, supporting signal transduction, and increasing antioxidant protection of the embryo, protects heat stressed maturing bovine oocyte and restores its developmental competence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.