ArticleInternational journal of molecular sciences2021
Degradation Products of Tryptophan in Cell Culture Media: Contribution to Color and Toxicity.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Integrating text mining and knowledge graph to enhance biopharmaceutical process optimization.PloS one · 2026Article
- Different Temperature Storage Conditions and Packaging Types Affects Colour Parameters, Amino Acid Composition, Microbial Contamination, and Key Bioactive Molecules ofMolecules (Basel, Switzerland) · 2025Article
- Metabolic studies of Ogataea polymorpha using nine different corn steep liquors.BMC biotechnology · 2025Article
- Cell specific perfusion rates drive growth dynamics and metabolism in CHO N-1 perfusion processes independent of perfusion rate control method.Frontiers in bioengineering and biotechnology · 2025Article
- Tryptophan Stability and Palatability in the Food Formulation: A Review.Current pharmaceutical biotechnology · 2025Review
- Stability and Requirement for Thiamin in a Cell Culture Feed Used to Produce New Biological Entities.Cells · 2023Article
- Tryptophan in Nutrition and Health.International journal of molecular sciences · 2022Article
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Authors and funding
7 authors.
Funding
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Abstract
Biomanufacturing processes may be optimized by storing cell culture media at room temperature, but this is currently limited by their instability and change in color upon long-term storage. This study demonstrates that one of the critical contributing factors toward media browning is tryptophan. LC-MS technology was utilized to identify tryptophan degradation products, which are likely formed primarily from oxidation reactions. Several of the identified compounds were shown to contribute significantly to color in solutions but also to exhibit toxicity against CHO cells. A cell-culture-compatible antioxidant, a-ketoglutaric acid, was found to be an efficient cell culture media additive for stabilizing components against degradation, inhibiting the browning of media formulations, and decreasing ammonia production, thus providing a viable method for developing room-temperature stable cell culture media.
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Registered trials
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