ArticleMaterials (Basel, Switzerland)2021
A Method for the Evaluation of Early Osseointegration of Implant Materials Ex Vivo: Human Bone Organ Model.
Article in Materials (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Oral Squamous Cell Carcinoma Associated with Dental Implants: A Literature Review with Focus on Field-Cancerized Mucosa.Cancers · 2025Review
- Culture system for longitudinal monitoring of bone dynamics ex vivo.Biotechnology and bioengineering · 2025Article
- An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro.International journal of molecular sciences · 2024Article
- Active osseointegration in anFrontiers in bioengineering and biotechnology · 2024Article
- Bioreactor analyses of tissue ingrowth, ongrowth and remodelling around implants: An alternative to live animal testing.Frontiers in bioengineering and biotechnology · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
In the present work, an ex vivo organ model using human bone (explant) was developed for the evaluation of the initial osseointegration behavior of implant materials. The model was tested with additive manufactured Ti6Al4V test substrates with different 3D geometries. Explants were obtained from patients who underwent total knee replacement surgery. The tibial plateaus were used within 24 h after surgery to harvest bone cylinders (BC) from the anterior side using hollow burrs. The BCs were brought into contact with the test substrate and inserted into an agarose mold, then covered with cell culture media and subjected to the external load of 500 g. Incubation was performed for 28 days. After 28d the test substrate was removed for further analysis. Cells grown out BC onto substrate were immunostained with DAPI and with an antibody against Collagen-I and alkaline phosphatase (ALP) for visualization and cell counting. We show that cells stayed alive for up to 28d in our organ model. The geometry of test substrates influences the number of cells grown onto substrate from BCs. The model presented here can be used for testing implant materials as an alternative for in vitro tests and animal models.
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Registered trials
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