Evidence map›Paper›PMID 34205481›Full record

ArticleCells2021

New Stable Cell Lines Derived from the Proximal and Distal Intestine of Rainbow Trout (

Rolando Pasquariello, Nicole Verdile, Radmila Pavlovic, Sara Panseri, Kristin Schirmer, Tiziana A L Brevini, Fulvio Gandolfi

Abstract read
In one paragraph

Article in Cells, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Development of a Rainbow Trout (Animals : an open access journal from MDPI · 2023
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Rolando PasquarielloDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy.ORCID 0000-0003-3191-7009
Nicole VerdileDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy.
Radmila PavlovicDepartment of Health, Animal Science and Food Safety, University of Milan, 20133 Milano, Italy.ORCID 0000-0002-2128-4589
Sara PanseriDepartment of Health, Animal Science and Food Safety, University of Milan, 20133 Milano, Italy.
Kristin SchirmerDepartment of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland.
Tiziana A L BreviniDepartment of Health, Animal Science and Food Safety, University of Milan, 20133 Milano, Italy.ORCID 0000-0002-0428-3621
Fulvio GandolfiDepartment of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy.ORCID 0000-0002-3246-2985

Funding

European Union's Horizon 2020 research and innovation program 828835
6 · The paper itself

Abstract

We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) and compared them with the previously established continuous cell line RTgutGC. Intestinal stem cells, differentiating and differentiated epithelial cells, and connective cells were found in all cell lines. The cell lines formed a polarized barrier, which was not permeable to large molecules and absorbed proline and glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by the downregulation of intestinal stem cell-related genes (i.e.,

Indexed as

EnterocytesAnimalsCell LineOncorhynchus mykisscell differentiationenterocyte differentiation markersfishintestinal barrier in vitrointestinal cell linesintestinal stem cellsnutrition

Identifiers

PMID34205481
PMCPMC8235179

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.