ArticleInternational journal of molecular sciences2021
Optogenetically Controlled Activity Pattern Determines Survival Rate of Developing Neocortical Neurons.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- Network integration of neocortical Cajal-Retzius neurons shapes early cortical dynamics and contributes to their transient nature.Cell communication and signaling : CCS · 2026Article
- Differentiation of cortical areas: effects of free energy minimization with broken symmetry.Cerebral cortex (New York, N.Y. : 1991) · 2026Article
- Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens.Poultry science · 2026Article
- Compensatory attenuation of cortical apoptosis by SK2 downregulation following ketamine anesthesia.Frontiers in pharmacology · 2026Article
- Early life shifts in cortical inhibitory-excitatory balance underlies sensitive periods and skill development.Frontiers in cellular neuroscience · 2026Article
- Development of spontaneous and sensory evoked network activity in rodent cerebral cortexFrontiers in cellular neuroscience · 2025Review
- Minimization of prediction errors during cerebral embryogenesis and the emergence of agency.Frontiers in systems neuroscience · 2025Article
- Cortical development in the structural model and free energy minimization.Cerebral cortex (New York, N.Y. : 1991) · 2024Article
- Transplantation of dorsal root ganglia overexpressing the NaChBac sodium channel improves locomotion after complete SCI.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Markov Blankets and Mirror Symmetries-Free Energy Minimization and Mesocortical Anatomy.Entropy (Basel, Switzerland) · 2024Article
- Human neuronal maturation comes of age: cellular mechanisms and species differences.Nature reviews. Neuroscience · 2024Review
- Activity-dependent regulation of the BAX/BCL-2 pathway protects cortical neurons from apoptotic death during early development.Cellular and molecular life sciences : CMLS · 2023Article
- Optogenetic stimulation shapes dendritic trees of infragranular cortical pyramidal cells.Frontiers in cellular neuroscience · 2023Article
- The mesoanatomy of the cortex, minimization of free energy, and generative cognition.Frontiers in computational neuroscience · 2023Article
- Large-scale waves of activity in the neonatal mouse brain in vivo occur almost exclusively during sleep cycles.Developmental neurobiology · 2022Article
- Calcium and activity-dependent signaling in the developing cerebral cortex.Development (Cambridge, England) · 2022Review
- Unification of free energy minimization, spatiotemporal energy, and dimension reduction models of V1 organization: Postnatal learning on an antenatal scaffold.Frontiers in computational neuroscience · 2022Article
- Spontaneous Activity Predicts Survival of Developing Cortical Neurons.Frontiers in cell and developmental biology · 2022Article
- Epigenetic and Transcriptional Regulation of Spontaneous and Sensory Activity Dependent Programs During Neuronal Circuit Development.Frontiers in neural circuits · 2022Review
- Gabaergic Interneurons in Early Brain Development: Conducting and Orchestrated by Cortical Network Activity.Frontiers in molecular neuroscience · 2021Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A substantial proportion of neurons undergoes programmed cell death (apoptosis) during early development. This process is attenuated by increased levels of neuronal activity and enhanced by suppression of activity. To uncover whether the mere level of activity or also the temporal structure of electrical activity affects neuronal death rates, we optogenetically controlled spontaneous activity of synaptically-isolated neurons in developing cortical cultures. Our results demonstrate that action potential firing of primary cortical neurons promotes neuronal survival throughout development. Chronic patterned optogenetic stimulation allowed to effectively modulate the firing pattern of single neurons in the absence of synaptic inputs while maintaining stable overall activity levels. Replacing the burst firing pattern with a non-physiological, single pulse pattern significantly increased cell death rates as compared to physiological burst stimulation. Furthermore, physiological burst stimulation led to an elevated peak in intracellular calcium and an increase in the expression level of classical activity-dependent targets but also decreased Bax/BCL-2 expression ratio and reduced caspase 3/7 activity. In summary, these results demonstrate at the single-cell level that the temporal pattern of action potentials is critical for neuronal survival versus cell death fate during cortical development, besides the pro-survival effect of action potential firing per se.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.