Evidence map›Paper›PMID 34204449›Full record

ArticleInternational journal of molecular sciences2021

The mTORC2 Regulator Homer1 Modulates Protein Levels and Sub-Cellular Localization of the CaSR in Osteoblast-Lineage Cells.

Mark S Rybchyn, Tara Clare Brennan-Speranza, David Mor, Zhiqiang Cheng, Wenhan Chang, Arthur D Conigrave, Rebecca S Mason

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. mTOR Signaling Network in Cell Biology and Human Disease.International journal of molecular sciences · 2022
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Mark S RybchynDiscipline of Physiology, School of Medical Sciences and Bosch Institute, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-4336-6227
Tara Clare Brennan-SperanzaDiscipline of Physiology, School of Medical Sciences and Bosch Institute, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0001-5472-4969
David MorDiscipline of Anatomy and Histology, School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.
Zhiqiang ChengSchool of Medicine, University of California, San Francisco, CA 94121, USA.
Wenhan ChangSchool of Medicine, University of California, San Francisco, CA 94121, USA.
Arthur D ConigraveSchool of Life and Environmental Science, Charles Perkins Centre (D17), University of Sydney, Sydney, NSW 2006, Australia.
Rebecca S MasonDiscipline of Physiology, School of Medical Sciences and Bosch Institute, Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0003-1534-9697
The University of Sydney · AUUniversity of California, San Francisco · US

Funding

UCSF Core Center for Musculoskeletal Biology and MedicineP30AR066262 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOTZ, JEFFREY C. · 2014 to 2018
$3.0M
BLRD VA I01 BX005851BLRD VA IK6 BX004835National Health and Medical Research Council 1008282NIAMS NIH HHS P30 AR066262
6 · The paper itself

Abstract

We recently found that, in human osteoblasts, Homer1 complexes to Calcium-sensing receptor (CaSR) and mediates AKT initiation via mechanistic target of rapamycin complex (mTOR) complex 2 (mTORC2) leading to beneficial effects in osteoblasts including β-catenin stabilization and mTOR complex 1 (mTORC1) activation. Herein we further investigated the relationship between Homer1 and CaSR and demonstrate a link between the protein levels of CaSR and Homer1 in human osteoblasts in primary culture. Thus, when siRNA was used to suppress the CaSR, we observed upregulated Homer1 levels, and when siRNA was used to suppress Homer1 we observed downregulated CaSR protein levels using immunofluorescence staining of cultured osteoblasts as well as Western blot analyses of cell protein extracts. This finding was confirmed in vivo as the bone cells from osteoblast specific

Indexed as

AnimalsCalciumCell LineageCells, CulturedCell SurvivalFemaleGene ExpressionGlycogen Synthase Kinase 3 betaHomer Scaffolding ProteinsHumansMaleMechanistic Target of Rapamycin Complex 2MiceMice, KnockoutOsteoblastsPhosphorylationCalciumCASR protein, humanGlycogen Synthase Kinase 3 betaHOMER1 protein, humanHomer Scaffolding ProteinsMechanistic Target of Rapamycin Complex 2Proto-Oncogene Proteins c-aktReceptors, Calcium-SensingAKTCaSRchaperone functionHomer1mTORosteoblastosteocyte

Identifiers

PMID34204449
PMCPMC8234890
OpenAlexW3168507128

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.