ArticleInternational journal of molecular sciences2021
UHPLC-ESI-MS/MS Quantification of Relevant Substrates and Metabolites of the Kynurenine Pathway Present in Serum and Peritoneal Fluid from Gastric Cancer Patients-Method Development and Validation.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- ssNetShift: single-sample metabolic network rewiring reveals hidden prognostic subtypes beyond clinical staging in gastric cancer.Briefings in bioinformatics · 2026Article
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- Association of maternal gut microbiota with prenatal depression and the potential mediation effect by plasma tryptophan.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2025Article
- Promising Gastric Cancer Biomarkers-Focus on Tryptophan Metabolism via the Kynurenine Pathway.International journal of molecular sciences · 2025Review
- A Novel Liquid Chromatography-Tandem Mass Spectrometry Method to Quantify Tryptophan and Its Major Metabolites in Serum to Support Biomarker Studies in Patients with Cancer Undergoing Immunotherapy.Molecules (Basel, Switzerland) · 2024Article
- 4-Vinylpyridine copolymers for improved LC-MS tryptophan and kynurenine determination in human serum.Scientific reports · 2024Article
- Dietary Kynurenine Pathway Metabolites-Source, Fate, and Chromatographic Determinations.International journal of molecular sciences · 2023Review
- Polarity-regulated derivatization-assisted LC-MS method for amino-containing metabolites profiling in gastric cancer.Journal of pharmaceutical analysis · 2023Article
- Helicobacter pylori promotes gastric intestinal metaplasia through activation of IRF3-mediated kynurenine pathway.Cell communication and signaling : CCS · 2023Article
- The Influence of Exercise Intensity on Tryptophan Metabolites in Thoroughbred Horses.Pharmaceuticals (Basel, Switzerland) · 2023Article
- The Role of Tryptophan Metabolites in Neuropsychiatric Disorders.International journal of molecular sciences · 2022Review
- The Kynurenine Pathway in Obese Middle-Aged Women with Normoglycemia and Type 2 Diabetes.Metabolites · 2022Article
- Kynurenine and Anthranilic Acid in the Peritoneum Correlate With the Stage of Gastric Cancer Disease.International journal of tryptophan research : IJTR · 2022Article
- Electrochemical Determination of Kynurenine Pathway Metabolites-Challenges and Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Kynurenine Pathway Metabolites as Potential Clinical Biomarkers in Coronary Artery Disease.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Metabolites and enzymes involved in the kynurenine pathway (KP) are highly promising targets for cancer treatment, including gastrointestinal tract diseases. Thus, accurate quantification of these compounds in body fluids becomes increasingly important. The aim of this study was the development and validation of the UHPLC-ESI-MS/MS methods for targeted quantification of biologically important KP substrates (tryptophan and nicotinamide) and metabolites(kynurenines) in samples of serum and peritoneal fluid from gastric cancer patients. The serum samples were simply pretreated with trichloroacetic acid to precipitate proteins. The peritoneal fluid was purified by solid-phase extraction before analysis. Validation was carried out for both matrices independently. Analysis of the samples from gastric cancer patients showed different accumulations of tryptophan and its metabolites in different biofluids of the same patient. The protocols will be used for the evaluation of tryptophan and kynurenines in blood and peritoneal fluid to determine correlation with the clinicopathological status of gastric cancer or the disease's prognosis.
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