Evidence map›Paper›PMID 34203429›Full record

ArticleInternational journal of molecular sciences2021

Level of Murine DDX3 RNA Helicase Determines Phenotype Changes of Hepatocytes In Vitro and In Vivo.

Olga Sergeeva, Tatiana Abakumova, Ilia Kurochkin, Renata Ialchina, Anna Kosyreva, Tatiana Prikazchikova, Varvara Varlamova, Evgeniya Shcherbinina, Timofei Zatsepin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Olga SergeevaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.ORCID 0000-0001-7883-9058
Tatiana AbakumovaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.
Ilia KurochkinSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.
Renata IalchinaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.ORCID 0000-0002-9508-853X
Anna KosyrevaResearch Institute of Human Morphology, 117418 Moscow, Russia.ORCID 0000-0002-6182-1799
Tatiana PrikazchikovaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.
Varvara VarlamovaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.
Evgeniya ShcherbininaSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.
Timofei ZatsepinSkolkovo Institute of Science and Technology, Skolkovo, 121205 Moscow, Russia.ORCID 0000-0003-0030-9174
Skolkovo Institute of Science and Technology · RUResearch Institute of Human Morphology · RU

Funding

Russian Science Foundation 19-74-00119
6 · The paper itself

Abstract

DDX3 RNA helicase is intensively studied as a therapeutic target due to participation in the replication of some viruses and involvement in cancer progression. Here we used transcriptome analysis to estimate the primary response of hepatocytes to different levels of RNAi-mediated knockdown of DDX3 RNA helicase both in vitro and in vivo. We found that a strong reduction of DDX3 protein (>85%) led to similar changes in vitro and in vivo-deregulation of the cell cycle and Wnt and cadherin pathways. Also, we observed the appearance of dead hepatocytes in the healthy liver and a decrease of cell viability in vitro after prolonged treatment. However, more modest downregulation of the DDX3 protein (60-65%) showed discordant results in vitro and in vivo-similar changes in vitro as in the case of strong knockdown and a different phenotype in vivo. These results demonstrate that the level of DDX3 protein can dramatically influence the cell phenotype in vivo and the decrease of DDX3, for more than 85% leads to cell death in normal tissues, which should be taken into account during the drug development of DDX3 inhibitors.

Indexed as

AnimalsCell SurvivalDEAD-box RNA HelicasesFemaleGene Expression Regulation, NeoplasticHepatocytesLiverMiceMice, Inbred BALB CTranscriptomeDdx3x protein, mouseDEAD-box RNA HelicasescancerliverRNA helicaseRNA therapytranscriptome

Identifiers

PMID34203429
PMCPMC8269429
OpenAlexW3174657045

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.