ArticleDiagnostics (Basel, Switzerland)2021
Post-Mortem Detection of SARS-CoV-2 RNA in Long-Buried Lung Samples.
Article in Diagnostics (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Burnout and Stress in Forensic Science Jobs: A Systematic Review.Healthcare (Basel, Switzerland) · 2024Review
- Unveiling the Pathological Mechanisms of Death Induced by SARS-CoV-2 Viral Pneumonia.Microorganisms · 2024Article
- Electrophysiological properties and structural prediction of the SARS-CoV-2 viroprotein E.Frontiers in molecular biosciences · 2024Article
- COVID-19: detection methods in post-mortem samples.Pathologica · 2023Review
- Substantial decrease in SARS-CoV-2 RNA after fixation of cadavers intended for anatomical dissection.Anatomical science international · 2023Article
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- SARS-CoV-2's high rate of genetic mutation under immune selective pressure: from oropharyngeal B.1.1.7 to intrapulmonary B.1.533 in a vaccinated patient.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Article
- Overview of the Clinical and Molecular Features of Legionella Pneumophila: Focus on Novel Surveillance and Diagnostic Strategies.Antibiotics (Basel, Switzerland) · 2022Review
- ACE2 protein expression in lung tissues of severe COVID-19 infection.Scientific reports · 2022Article
- The Impact of the COVID-19 Pandemic on the Practice of Forensic Medicine: An Overview.Healthcare (Basel, Switzerland) · 2022Review
- Host cell entry mediators implicated in the cellular tropism of SARS‑CoV‑2, the pathophysiology of COVID‑19 and the identification of microRNAs that can modulate the expression of these mediators (Review).International journal of molecular medicine · 2022Review
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- Evidence of SARS-CoV-2 Antibodies and RNA on Autopsy Cases in the Pre-Pandemic Period in Milan (Italy).Frontiers in microbiology · 2022Article
- Pathological Findings Associated With SARS-CoV-2 on Postmortem Core Biopsies: Correlation With Clinical Presentation and Disease Course.Frontiers in medicine · 2022Article
- Low Represented Mutation Clustering in SARS-CoV-2 B.1.1.7 Sublineage Group with Synonymous Mutations in the E Gene.Diagnostics (Basel, Switzerland) · 2021Article
- Pharmacogenetics and Forensic Toxicology: A New Step towards a Multidisciplinary Approach.Toxics · 2021Review
- COVID-19 Pneumonia Detection Using Optimized Deep Learning Techniques.Diagnostics (Basel, Switzerland) · 2021Article
- Do We Really Need Hazard Prevention at the Expense of Safeguarding Death Dignity in COVID-19?Diagnostics (Basel, Switzerland) · 2021Article
- Dying "from" or "with" COVID-19 during the Pandemic: Medico-Legal Issues According to a Population Perspective.International journal of environmental research and public health · 2021Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Coronavirus Disease 19 (COVID-19) pandemic has caused an unexpected death toll worldwide. Even though several guidelines for the management of infectious corpses have been proposed, the limited number of post-mortem analyses during the pandemic has led to inaccuracies in the counting of COVID-19 deaths and contributed to a lack of important information about the pathophysiology of the SARS-CoV-2 infection. Due to the impossibility of carrying out autopsies on all corpses, the scientific community has raised the question of whether confirmatory analyses could be performed on exhumed bodies after a long period of burial to assess the presence of SARS-CoV-2 RNA. Post-mortem lung samples were collected from 16 patients who died from COVID-19 infection and were buried for a long period of time. A custom RNA extraction protocol was developed to enhance extraction of viral RNA from degraded samples and highly sensitive molecular methods, including RT-qPCR and droplet digital PCR (ddPCR), were used to detect the presence of SARS-CoV-2 RNA. The custom extraction protocol developed allowed us to extract total RNA effectively from all lung samples collected. SARS-CoV-2 viral RNA was effectively detected in all samples by both RT-qPCR and ddPCR, regardless of the length of burial. ddPCR results confirmed the persistence of the virus in this anatomical niche and revealed high viral loads in some lung samples, suggesting active infection at the time of death. To the best of our knowledge, this is the first study to demonstrate the persistence of SARS-CoV-2 viral RNA in the lung even after a long post-mortem interval (up to 78 days). The extraction protocol herein described, and the highly sensitive molecular analyses performed, could represent the standard procedures for SARS-CoV-2 detection in degraded lung specimens. Finally, the innovative results obtained encourage post-mortem confirmatory analyses even after a long post-mortem interval.
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