Evidence map›Paper›PMID 34201211›Full record

ArticleCurrent issues in molecular biology2021

Three-Dimensional Reconstructed Bone Marrow Matrix Culture Improves the Viability of Primary Myeloma Cells In-Vitro via a STAT3-Dependent Mechanism.

Yung-Hsing Huang, Meaad Almowaled, Jing Li, Christopher Venner, Irwindeep Sandhu, Anthea Peters, Afsaneh Lavasanifar, Raymond Lai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Yung-Hsing HuangDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Meaad AlmowaledDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Jing LiDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Christopher VennerDepartment of Oncology, University of Alberta, Edmonton, AB T6G 2R7, Canada.
Irwindeep SandhuDepartment of Oncology, University of Alberta, Edmonton, AB T6G 2R7, Canada.
Anthea PetersDepartment of Oncology, University of Alberta, Edmonton, AB T6G 2R7, Canada.ORCID 0000-0002-2570-339X
Afsaneh LavasanifarFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G2H7, Canada.
Raymond LaiDepartment of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2E1, Canada.ORCID 0000-0002-6963-2407
University of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary myeloma (PM) cells are short-lived in conventional culture, which limited their usefulness as a study model. Here, we evaluated if three-dimensional (3D) culture can significantly prolong the longevity of PM cells in-vitro. We employed a previously established 3D model for culture of bone marrow mononuclear cells isolated from 15 patients. We assessed the proportion of PM cells, viability and proliferation using CD38 staining, trypan blue exclusion assays and carboxy fluorescein succinimidyl ester (CFSE) staining, respectively. We observed significantly more CD38+ viable cells in 3D than in conventional culture (65% vs. 25%,

Indexed as

Cell Culture TechniquesCell SurvivalADP-ribosyl Cyclase 1AgedBone MarrowCell ProliferationCells, CulturedCyclic S-OxidesFemaleFluoresceinsHumansIn Vitro TechniquesLeukocytes, MononuclearMaleMembrane GlycoproteinsMiddle Aged5-(6)-carboxyfluorescein diacetate succinimidyl esterADP-ribosyl Cyclase 1CD38 protein, humanCyclic S-OxidesFluoresceinsMembrane GlycoproteinsSTAT3 protein, humanSTAT3 Transcription FactorstatticSuccinimides3D cultureprimary myeloma cellsSTAT3

Identifiers

PMID34201211
PMCPMC8928965
OpenAlexW3168451900

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.