ReviewInternational journal of molecular sciences2021
Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies.
Review in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Hydrogel Systems in Plant Germplasm Cryopreservation: A Comprehensive Review.Gels (Basel, Switzerland) · 2026Review
- Long-Term Preservation by the Cryopreservation Method of Swietenia humilis Zucc.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Enhancing Cryopreservation Efficiency inPlants (Basel, Switzerland) · 2025Article
- Alginate Hydrogel with Pluronic F-68 Enhances Cryopreservation Efficiency in Peach Germplasm.Gels (Basel, Switzerland) · 2025Article
- Optimizing in vitro slow-growth conservation media for garlic under ambient conditions: further implications for core set accessions.BMC plant biology · 2025Article
- Trends and Challenges in Plant Cryopreservation Research: A Meta-Analysis of Cryoprotective Agent Development and Research Focus.Plants (Basel, Switzerland) · 2025Review
- The role of heat shock protein 70 on oocyte apoptosis during vitrification.Open veterinary journal · 2025Review
- Comparative analysis ofFrontiers in pharmacology · 2025Article
- Endophytic bacterial community dynamics in sweet cherry in vitro shoot culture and their role in shoot adaptation after cryopreservation.BMC plant biology · 2024Article
- Improvement and Innovation of Cryopreservation and In Vitro Methods in Plant Resource Protection.Biology · 2024Article
- Cryopreservation of Duckweed Genetic Diversity as Model for Long-Term Preservation of Aquatic Flowering Plants.Plants (Basel, Switzerland) · 2023Article
- A Comprehensive Review Uncovering the Challenges and Advancements in the In Vitro Propagation ofPlants (Basel, Switzerland) · 2023Review
- Tracking Permeation of Dimethyl Sulfoxide (DMSO) inPlants (Basel, Switzerland) · 2023Article
- Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement.Plant methods · 2023Review
- Critical Role of Regrowth Conditions in Post-Cryopreservation of In Vitro Plant Germplasm.Biology · 2023Review
- Cryopreservation of bioflavonoid-rich plant sources and bioflavonoid-microcapsules: emerging technologies for preserving bioactivity and enhancing nutraceutical applications.Frontiers in nutrition · 2023Review
- Article
- Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions.Biology · 2022Review
- Plant Cell and Organism Development 2.0.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (-135 °C to -196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.
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