Evidence map›Paper›PMID 34199780›Full record

ArticleBrain sciences2021

Three-Dimensional X-ray Imaging of β-Galactosidase Reporter Activity by Micro-CT: Implication for Quantitative Analysis of Gene Expression.

Olga Ermakova, Tiziana Orsini, Paolo Fruscoloni, Francesco Chiani, Alessia Gambadoro, Sabrina Putti, Maurizio Cirilli, Alessio Mezzi, Saulius Kaciulis, Miriam Pasquini and 3 more

Open access · goldAbstract read
In one paragraph

Article in Brain sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. TheCells · 2024
    Article
  2. Gene expression detection in developing mouse tissue using in situ hybridization and µCT imaging.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  3. Article
  4. Article
  5. Article
  6. Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Olga ErmakovaAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Tiziana OrsiniAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.ORCID 0000-0003-0023-7916
Paolo FruscoloniAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.ORCID 0000-0001-5483-4754
Francesco ChianiAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Alessia GambadoroAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Sabrina PuttiAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Maurizio CirilliAdriano Buzzati-Traverso Campus, Institute of Biochemistry and Cell Biology (IBBC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo Scalo, 00015 Rome, Italy.
Alessio MezziInstitute for the Study of Nanostructured Materials, ISMN-CNR, Monterotondo Staz., 00015 Rome, Italy.ORCID 0000-0002-2887-520X
Saulius KaciulisInstitute for the Study of Nanostructured Materials, ISMN-CNR, Monterotondo Staz., 00015 Rome, Italy.ORCID 0000-0002-9868-7626
Miriam PasquiniAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Marcello RaspaAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Ferdinando ScavizziAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
Glauco P Tocchini-ValentiniAdriano Buzzati-Traverso Campus, European Mouse Mutant Archive (EMMA), INFRAFRONTIER, Monterotondo Mouse Clinic (MMC), Italian National Research Council (CNR), Via Ramarini, 32, Monterotondo, 00015 Rome, Italy.
National Research Council · ITInstitute of Nanostructured Materials · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution in minimally perturbed naive non-transparent samples has recently gained increased popularity and broad application in biomedical research. Here, we describe a novel X-ray-based methodology for analysis of

Indexed as

gene expressionlacZ reportermouse brainmouse phenotypingpontocerebellar hypoplesiaTsen54X-ray imaging

Identifiers

PMID34199780
PMCPMC8230009
OpenAlexW3170876189

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.