ArticleAnimals : an open access journal from MDPI2021
A Grain-Based SARA Challenge Affects the Composition of Epimural and Mucosa-Associated Bacterial Communities throughout the Digestive Tract of Dairy Cows.
Article in Animals : an open access journal from MDPI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Yak Gut Microbiota: A Systematic Review and Meta-Analysis.Frontiers in veterinary science · 2022Pooled it
- Subacute ruminal acidosis in high-producing dairy cows: an integrated diet-microbiota-epithelium-host framework for diagnosis and nutritional management.Journal of animal science and biotechnology · 2026Review
- Plant extracts to enhancing intestinal barrier function in fattening lambs: review.Animal bioscience · 2026Article
- Microbial landscapes in dairy cow diseases: from localized dysbiosis to inter-organ axes.NPJ biofilms and microbiomes · 2026Review
- Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows.Animal microbiome · 2026Article
- Alkaline mineral complex supplementation alters gut microbiota and metabolic profiles and supports colonic health in finishing cattle.Frontiers in microbiology · 2026Article
- Rumen acidosis in ruminants: a review of the effects of high-concentrate diets and the potential modulatory role of rumen foam.Frontiers in veterinary science · 2025Review
- Macrogenomics-based analysis of rumen microbial composition and their metabolic pathways in yaks under different dietary concentrate-to-forage ratios.Frontiers in microbiology · 2025Article
- Article
- Host Species Affects Bacterial Evenness, but Not Diversity: Comparison of Fecal Bacteria of Cows and Goats Offered the Same Diet.Animals : an open access journal from MDPI · 2022Article
- Differential Responses of Digesta- and Mucosa-Associated Jejunal Microbiota of Hu Sheep to Pelleted and Non-Pelleted High-Grain Diets.Animals : an open access journal from MDPI · 2022Article
- Dietary forage to concentrate ratios impact on yak ruminal microbiota and metabolites.Frontiers in microbiology · 2022Article
- Effects of supplementation of sodium acetate on rumen fermentation and microbiota in postpartum dairy cows.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
The effects of a subacute ruminal acidosis (SARA) challenge on the composition of epimural and mucosa-associated bacterial communities throughout the digestive tract were determined in eight non-lactating Holstein cows. Treatments included feeding a control diet containing 19.6% dry matter (DM) starch and a SARA-challenge diet containing 33.3% DM starch for two days after a 4-day grain step-up. Subsequently, epithelial samples from the rumen and mucosa samples from the duodenum, proximal, middle and distal jejunum, ileum, cecum and colon were collected. Extracted DNA from these samples were analyzed using MiSeq Illumina sequencing of the V4 region of the 16S rRNA gene. Distinct clustering patterns for each diet existed for all sites. The SARA challenge decreased microbial diversity at all sites, with the exception of the middle jejunum. The SARA challenge also affected the relative abundances of several major phyla and genera at all sites but the magnitude of these effects differed among sites. In the rumen and colon, the largest effects were an increase in the relative abundance of Firmicutes and a reduction of Bacteroidetes. In the small intestine, the largest effect was an increase in the relative abundance of Actinobacteria. The grain-based SARA challenge conducted in this study did not only affect the composition and cause dysbiosis of epimural microbiota in the rumen, it also affected the mucosa-associated microbiota in the intestines. To assess the extent of this dysbiosis, its effects on the functionality of these microbiota must be determined in future.
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Registered trials
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