Evidence map›Paper›PMID 34197621›Full record

ArticleNucleic acids research2021

TRAPID 2.0: a web application for taxonomic and functional analysis of de novo transcriptomes.

François Bucchini, Andrea Del Cortona, Łukasz Kreft, Alexander Botzki, Michiel Van Bel, Klaas Vandepoele

Abstract read
In one paragraph

Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

François BucchiniDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.ORCID 0000-0002-5686-7960
Andrea Del CortonaDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Łukasz KreftVIB Bioinformatics Core, VIB, 9052 Ghent, Belgium.
Alexander BotzkiVIB Bioinformatics Core, VIB, 9052 Ghent, Belgium.
Michiel Van BelDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.ORCID 0000-0002-1873-2563
Klaas VandepoeleDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.ORCID 0000-0003-4790-2725

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in high-throughput sequencing have resulted in a massive increase of RNA-Seq transcriptome data. However, the promise of rapid gene expression profiling in a specific tissue, condition, unicellular organism or microbial community comes with new computational challenges. Owing to the limited availability of well-resolved reference genomes, de novo assembled (meta)transcriptomes have emerged as popular tools for investigating the gene repertoire of previously uncharacterized organisms. Yet, despite their potential, these datasets often contain fragmented or contaminant sequences, and their analysis remains difficult. To alleviate some of these challenges, we developed TRAPID 2.0, a web application for the fast and efficient processing of assembled transcriptome data. The initial processing phase performs a global characterization of the input data, providing each transcript with several layers of annotation, comprising structural, functional, and taxonomic information. The exploratory phase enables downstream analyses from the web application. Available analyses include the assessment of gene space completeness, the functional analysis and comparison of transcript subsets, and the study of transcripts in an evolutionary context. A comparison with similar tools highlights TRAPID's unique features. Finally, analyses performed within TRAPID 2.0 are complemented by interactive data visualizations, facilitating the extraction of new biological insights, as demonstrated with diatom community metatranscriptomes.

Indexed as

Web BrowserAmino Acid SequenceAnimalsClassificationComputational BiologyEvolution, MolecularGene Expression ProfilingGene OntologyHumansMolecular Sequence AnnotationPhylogenyReproducibility of ResultsRNA-SeqSequence Homology, Amino AcidSpecies Specificity

Identifiers

PMID34197621
PMCPMC8464036

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.