ArticlePLoS neglected tropical diseases2021
Optimizing the microscopic agglutination test (MAT) panel for the diagnosis of Leptospirosis in a low resource, hyper-endemic setting with varied microgeographic variation in reactivity.
Article in PLoS neglected tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- A recombinant Loa22-based latex agglutination test and indirect ELISA for the sero-detection of leptospirosis in dogs and cattle.Archives of microbiology · 2026Article
- Subclinical Leptospiral Infections and Antibody Waning During Pregnancy: A Population-Based Cohort Study from Rural Sri Lanka.The American journal of tropical medicine and hygiene · 2026Article
- Retrospective Molecular Detection and Characterization of PathogenicTropical medicine and infectious disease · 2026Article
- Genomic analysis of human-infecting Leptospira borgpetersenii isolates in Sri Lanka: Expanded PF07598 gene family repertoire and less genome reduction than bovine isolates.PLoS neglected tropical diseases · 2026Article
- Comprehensive genotyping and taxonomic analysis uncovers extensive distribution of intermediate Leptospira species in Colombia.World journal of microbiology & biotechnology · 2026Article
- Evaluation of the conserved subunit of the pathogenicMicrobiology spectrum · 2026Article
- Leptospirosis in the Caribbean Region between 2000 and 2022: A scoping review of morbidity and mortality.PLoS neglected tropical diseases · 2026Article
- The Role of Hydrometeorological Factors in Leptospirosis Transmission in Central Java, Indonesia.Journal of preventive medicine and public health = Yebang Uihakhoe chi · 2025Article
- Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.Microorganisms · 2025Review
- Leptospirosis in humans and animals in Malaysia: A review from 1976 to 2023.Veterinary world · 2025Review
- Review
- Evaluation of Serological Tests for Different Disease Stages of Leptospirosis Infection in Humans.Tropical medicine and infectious disease · 2024Article
- Development of a new accurate lateral flow immunoassay for diagnosis of human leptospirosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2024Article
- Navigating cross-reactivity and host species effects in a serological assay: A case study of the microscopic agglutination test for Leptospira serology.PLoS neglected tropical diseases · 2024Article
- Improved Antibody Detection for Canine Leptospirosis: ELISAs Modified Using Local Leptospiral Serovar Isolates from Asymptomatic Dogs.Animals : an open access journal from MDPI · 2024Article
- Levels of Cytokines in Leptospirosis Patients with Different Serovars andJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2024Article
- Oliguria as a diagnostic marker of severe leptospirosis: a study from the Transcarpathian region of Ukraine.Frontiers in cellular and infection microbiology · 2024Article
- Article
- Surveillance of human leptospirosis infections in Ukraine between 2018 and 2023.Frontiers in public health · 2024Article
- Uveitis and blindness in a closed herd of Equidae following leptospiral infection.Frontiers in veterinary science · 2024Article
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7 authors at 3 institutions in 2 countries.
Funding
Abstract
The microscopic agglutination test (MAT) is the standard serological reference test for the diagnosis of leptospirosis, despite being a technically demanding and laborious procedure. The use of a locally optimised MAT panel is considered essential for proper performance and interpretation of results. This paper describes the procedure of selecting such an optimised panel for Sri Lanka, a country hyper-endemic for leptospirosis. MAT was performed using 24 strains on 1132 serum samples collected from patients presenting with acute undifferentiated fever. Of 24 strains, 15 were selected as the optimised panel, while only 11% of serum samples showed positivity. A geographical variation in predominantly reactive serovars was observed, whereas reactivity was low with the saprophytic strain Patoc. Testing with paired sera yielded a higher sensitivity but provided only a retrospective diagnosis. Serological tests based on ELISA with complementary molecular diagnosis using PCR are a feasible and robust alternative approach to diagnose leptospirosis in countries having a higher burden of the disease.
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