ArticleAdvanced healthcare materials2021
Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Produce Distinct Neural 3D In Vitro Models Depending on Alginate/Gellan Gum/Laminin Hydrogel Blend Properties.
Article in Advanced healthcare materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Physical crowding in 3D niche regulates neural stem cell differentiation via Cx43-mediated gap junction communication.iScience · 2026Article
- Advanced Stem Cell Therapy: 3D-Bioprinted Brain-Like Transplants for Alzheimer's Disease-Like Dementia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Spheroid-Hydrogel-Integrated Biomimetic System: A New Frontier in Advanced Three-Dimensional Cell Culture Technology.Cells, tissues, organs · 2025Review
- Biomaterials for neuroengineering: applications and challenges.Regenerative biomaterials · 2025Review
- A polysaccharide gel made of gellan gum improves oocyte maturation and embryonic development in pigs.The Journal of reproduction and development · 2024Article
- Article
- Biopolymers for Tissue Engineering: Crosslinking, Printing Techniques, and Applications.Gels (Basel, Switzerland) · 2023Review
- Tunable hydrogel viscoelasticity modulates human neural maturation.Science advances · 2023Article
- Modeling development using microfluidics: bridging gaps to foster fundamental and translational research.Current opinion in genetics & development · 2023Review
- 3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regeneration.Bioactive materials · 2023Article
- Practical guide for preparation, computational reconstruction and analysis of 3D human neuronal networks in control and ischaemic conditions.Development (Cambridge, England) · 2022Article
- Tuning Polymer Hydrophilicity to Regulate Gel Mechanics and Encapsulated Cell Morphology.Advanced healthcare materials · 2022Article
- A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications.International journal of molecular sciences · 2022Review
- Induced Pluripotent Stem Cells for Treatment of Alzheimer's and Parkinson's Diseases.Biomedicines · 2022Review
- Alzheimer's Disease: Current Perspectives and Advances in Physiological Modeling.Bioengineering (Basel, Switzerland) · 2021Review
- A biopolymer hydrogel electrostatically reinforced by amino-functionalized bioactive glass for accelerated bone regeneration.Science advances · 2021Article
- Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Produce Distinct Neural 3D In Vitro Models Depending on Alginate/Gellan Gum/Laminin Hydrogel Blend Properties.Advanced healthcare materials · 2021Article
- Co-grafts of Human Embryonic Stem Cell Derived Retina Organoids and Retinal Pigment Epithelium for Retinal Reconstruction in Immunodeficient Retinal Degenerate Royal College of Surgeons Rats.Frontiers in neuroscience · 2021Article
- Development and Application of 3D Bioprinted Scaffolds Supporting Induced Pluripotent Stem Cells.BioMed research international · 2021Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable and predictive neural cell culture models are a necessary premise for many research fields. However, conventional 2D models lack 3D cell-material/-cell interactions and hence do not reflect the complexity of the in vivo situation properly. Here two alginate/gellan gum/laminin (ALG/GG/LAM) hydrogel blends are presented for the fabrication of human induced pluripotent stem cell (hiPSC)-based 3D neural models. For hydrogel embedding, hiPSC-derived neural progenitor cells (hiNPCs) are used either directly or after 3D neural pre-differentiation. It is shown that stiffness and stress relaxation of the gel blends, as well as the cell differentiation strategy influence 3D model development. The embedded hiNPCs differentiate into neurons and astrocytes within the gel blends and display spontaneous intracellular calcium signals. Two fit-for-purpose models valuable for i) applications requiring a high degree of complexity, but less throughput, such as disease modeling and long-term exposure studies and ii) higher throughput applications, such as acute exposures or substance screenings are proposed. Due to their wide range of applications, adjustability, and printing capabilities, the ALG/GG/LAM based 3D neural models are of great potential for 3D neural modeling in the future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.