ArticleAMB Express2021
Use of dual priming oligonucleotide system-based multiplex RT-PCR assay to detect five diarrhea viruses in pig herds in South China.
Article in AMB Express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Clinical applications and methodology updates in HLA typing.BMC immunology · 2026Review
- Epidemiological characteristics of co-infection between porcine epidemic diarrhea virus (PEDV) and other pathogens: a meta-analysis and systematic review.Porcine health management · 2026Article
- Swine Enteric Coronaviruses: An Updated Overview of Epidemiology, Diagnosis, Prevention, and Control.Animals : an open access journal from MDPI · 2026Review
- Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibody.Virology journal · 2025Article
- Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods.Discover nano · 2025Review
- Isolation, identification of porcine a rotavirus, and preparation of the monoclonal antibodies.Frontiers in veterinary science · 2025Article
- Establishment of a novel double-antibody sandwich fluorescence microsphere immunochromatographic test strip for rapid detection of swine acute diarrhea syndrome coronavirus (SADS-CoV) infection.Frontiers in cellular and infection microbiology · 2025Article
- Development and Clinical Application of a Molecular Assay for Four Common Porcine Enteroviruses.Veterinary sciences · 2024Article
- New insight in molecular detection of Mycobacterium tuberculosis.AMB Express · 2024Review
- Seroprevalence of the novel swine acute diarrhea syndrome coronavirus in China assessed by enzyme-linked immunosorbent assay.Frontiers in cellular and infection microbiology · 2024Article
- Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses.Pathogens (Basel, Switzerland) · 2023Article
- Development of a Multiplex Quantitative PCR for Detecting Porcine Epidemic Diarrhea Virus, Transmissible Gastroenteritis Virus, and Porcine Deltacoronavirus Simultaneously in China.Veterinary sciences · 2023Article
- Development of a multiplex qRT-PCR assay for the detection of porcine epidemic diarrhea virus, porcine transmissible gastroenteritis virus and porcine Deltacoronavirus.Frontiers in veterinary science · 2023Article
- A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses.Veterinary sciences · 2022Article
- Development of Duplex LAMP Technique for Detection of Porcine Epidemic Diarrhea Virus (PEDV) and Porcine Circovirus Type 2 (PCV 2).Current issues in molecular biology · 2022Article
- Establishment of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of swine acute diarrhea syndrome coronavirus (SADS-CoV).BMC veterinary research · 2022Article
- Current State of Molecular and Serological Methods for Detection of Porcine Epidemic Diarrhea Virus.Pathogens (Basel, Switzerland) · 2022Review
- Development of a multiplex RT-PCR method for the detection of four porcine enteric coronaviruses.Frontiers in veterinary science · 2022Article
- PEAC: An Ultrasensitive and Cost-Effective MRD Detection System in Non-small Cell Lung Cancer Using Plasma Specimen.Frontiers in medicine · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 2 countries.
Funding
Abstract
In this study, a specific and simple method based on the dual priming oligonucleotide (DPO) system was developed to simultaneously detect transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine rotavirus A (PRV-A), porcine delta coronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV), associated with the major enteric RNA viruses in pigs. The DPO system-based multiplex RT-PCR method simplified the primer design and did not require optimization of the annealing temperature. Specificity analysis revealed that the method could specifically detect TGEV, PEDV, PRV-A, PDCoV, and SADS-CoV without any cross-amplification of other circulating swine viruses. The limit of detection of the method was as low as 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.