ArticleMedical microbiology and immunology2021
Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings.
Article in Medical microbiology and immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal.Biosafety and health · 2025Article
- Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach.Heliyon · 2024Article
- Heat inactivation of SARS-CoV 2 enabled the measurement of salivary cortisol during COVID-19 pandemic.Endocrine · 2024Article
- Current status of pathogen handling in European laboratories: focus on viral inactivation process.Frontiers in bioengineering and biotechnology · 2024Article
- Ammonium sulfate denatures transport medium less dependent on guanidinium isothiocyanate and enables SARS-CoV-2 RNA and antigen detection compatibility.Frontiers in microbiology · 2024Article
- Analysis of SARS-CoV-2 interactions with the Vero cell lines by scanning electron microscopy.Journal of biological physics · 2023Article
- Article
- A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung.Experimental & molecular medicine · 2023Article
- Wavelength dependence of ultraviolet light inactivation for SARS-CoV-2 omicron variants.Scientific reports · 2023Article
- Article
- Preparation of noninfectious scRNAseq samples from SARS-CoV-2-infected epithelial cells.PloS one · 2023Article
- Hypoxia-altered cholesterol homeostasis enhances the expression of interferon-stimulated genes upon SARS-CoV-2 infections in monocytes.Frontiers in immunology · 2023Article
- NVX-CoV2373-induced cellular and humoral immunity towards parental SARS-CoV-2 and VOCs compared to BNT162b2 and mRNA-1273-regimens.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2022Observational
- Enhanced Inactivation of Pseudoparticles Containing SARS-CoV-2 S Protein Using Magnetic Nanoparticles and an Alternating Magnetic Field.ACS applied bio materials · 2022Article
- Wastewater surveillance allows early detection of SARS-CoV-2 omicron in North Rhine-Westphalia, Germany.The Science of the total environment · 2022Article
- Article
- Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments.Viruses · 2022Article
- Article
- Limited neutralisation of the SARS-CoV-2 Omicron subvariants BA.1 and BA.2 by convalescent and vaccine serum and monoclonal antibodies.EBioMedicine · 2022Article
- Sample Buffer Containing Guanidine-Hydrochloride Combines Biological Safety and RNA Preservation for SARS-CoV-2 Molecular Diagnostics.Diagnostics (Basel, Switzerland) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The novel coronavirus SARS-CoV-2 is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. Laboratory work with SARS-CoV-2 in a laboratory setting was rated to biosafety level 3 (BSL-3) biocontainment level. However, certain research applications in particular in molecular biology require incomplete denaturation of the proteins, which might cause safety issues handling contaminated samples. In this study, we evaluated lysis buffers that are commonly used in molecular biological laboratories for their ability to inactivate SARS-CoV-2. In addition, viral stability in cell culture media at 4 °C and on display glass and plastic surfaces used in laboratory environment was analyzed. Furthermore, we evaluated chemical and non-chemical inactivation methods including heat inactivation, UV-C light, addition of ethanol, acetone-methanol, and PFA, which might be used as a subsequent inactivation step in the case of insufficient inactivation. We infected susceptible Caco-2 and Vero cells with pre-treated SARS-CoV-2 and determined the tissue culture infection dose 50 (TCID
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.