Evidence map›Paper›PMID 34196749›Full record

ArticleArchives of microbiology2021

Application of MALDI-TOF mass spectrometry, and DNA sequencing-based SLST and MLST analysis for the identification of Cronobacter spp. isolated from environmental surveillance samples.

Irshad M Sulaiman, Kevin Tang, Katharine Segars, Nancy Miranda, Nikhat Sulaiman, Steven Simpson

Abstract read
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In one paragraph

Article in Archives of microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Identification, Typing and Drug Resistance ofFoods (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Irshad M SulaimanMicrobiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA, 30309, USA. Irshad.Sulaiman@fda.hhs.gov.ORCID http://orcid.org/0000-0001-7677-6129
Kevin TangBiotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, U.S. Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA, 30329, USA.
Katharine SegarsMicrobiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA, 30309, USA.
Nancy MirandaMicrobiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA, 30309, USA.
Nikhat SulaimanBiotechnology Core Facility Branch, Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, U.S. Centers for Disease Control and Prevention, 1600 Clifton Road, NE, Atlanta, GA, 30329, USA.
Steven SimpsonMicrobiological Sciences Branch, Southeast Food and Feed Laboratory, Office of Regulatory Science, Office of Regulatory Affairs, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA, 30309, USA.
United States Food and Drug Administration · USCenters for Disease Control and Prevention · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cronobacter spp. are emerging infectious foodborne bacteria that can cause acute meningitis and necrotizing enterocolitis in neonates and immunocompromised individuals. Although, little is known about its reservoirs or transmission routes, it has been linked to powdered infant formula worldwide. Three Cronobacter spp. (C. sakazakii, C. malonaticus, and C. turicensis) have been described as more virulent, and isolated frequently from infant meningitis cases. The estimated mortality rates are as high as 80% in infants. Thus, surveillance and typing of Cronobacter spp. isolated from food and environmental samples is essential to prevent contamination and spread of this pathogen. In this study, we have characterized 83 Cronobacter isolates recovered from various environmental samples by conventional microbiologic protocols. Species identification was accomplished by VITEK 2 system and real-time PCR analysis. Subsequently, these isolates were analyzed using VITEK MS system. Single locus sequence typing (SLST) was achieved by characterizing the regions of 16S rRNA and rpoB genes. Multilocus sequence typing (MLST) was performed by sequence characterization of seven housekeeping genes (atpD, fusA, glnS, gltB, gyrB, infB, and pps) using ABI 3500XL Genetic Analyzer. VITEK MS system identified, the majority of isolates as Cronobacter sakazakii with a high confidence value (99.9%). MLST analysis ascertained 12 distinct clonal complexes (CC1, CC4, CC8, CC13, CC17, CC21, CC31, CC40, CC52, CC64, CC73, and CC83) for the recovered C. sakazakii isolates. The results suggest that the MALDI-TOF MS is a reliable diagnostic tool for rapid species identification whereas 7-loci MLST is a powerful technique to discriminate and differentiate Cronobacter spp. isolates.

Indexed as

CronobacterCronobacter sakazakiiEnvironmental MonitoringFood MicrobiologyHumansInfantInfant, NewbornMultilocus Sequence TypingRNA, Ribosomal, 16SSequence Analysis, DNASpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationRNA, Ribosomal, 16SEnvironmental surveillanceFoodborne bacteriaGenetic polymorphismMALDI-TOF MSMLSTSLSTSpecies identification

Identifiers

PMID34196749
OpenAlexW3176926944

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.