ArticleThe Plant cell2021
Real-time monitoring of subcellular H2O2 distribution in Chlamydomonas reinhardtii.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 83 citations in OpenAlex.
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- Responsive Membrane Antenna Enables Adaptive Biohybrid Interface for Photosynthesis Promotion, Stress Reporting and Modulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Plasmodesmata display dynamic local and systemic redox responses during plant stress.The Plant cell · 2026Article
- The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast.Plant biology (Stuttgart, Germany) · 2026Article
- Reversible phase dynamics of PsAF5 regulate mitophagy to balance redox levels inScience advances · 2026Article
- Mitochondrial ROS trigger interorganellular signaling and prime ER processes to establish enhanced plant immunity.Science advances · 2025Article
- Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early PSII assembly factor.The Plant cell · 2025Article
- Abiotic Stress-Induced Chloroplast and Cytosolic CaPlant, cell & environment · 2025Article
- Simultaneous recording of biosensors and chlorophyll fluorescence reveals a tight correlation between ETR and oxidative signals.Plant physiology · 2025Article
- A Biohydrogel-Enabled Microneedle Sensor for In Situ Monitoring of Reactive Oxygen Species in Plants.ACS sensors · 2025Article
- Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Characteristics of RecombinantJournal of microbiology and biotechnology · 2024Article
- Singlet-Oxygen-Mediated Regulation of Photosynthesis-Specific Genes: A Role for Reactive Electrophiles in Signal Transduction.International journal of molecular sciences · 2024Article
- Activity-dependent mitochondrial ROS signaling regulates recruitment of glutamate receptors to synapses.eLife · 2024Article
- Optimized transgene expression in the red alga Porphyridium purpureum and efficient recombinant protein secretion into the culture medium.Plant molecular biology · 2024Article
- A Modular Cloning Toolkit for the production of recombinant proteins inMicrobial cell (Graz, Austria) · 2024Article
- TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response.Plant physiology · 2023Article
- Novel insight into functions of ascorbate peroxidase in higher plants: More than a simple antioxidant enzyme.Redox biology · 2023Review
- Current Nuclear Engineering Strategies in the Green MicroalgaLife (Basel, Switzerland) · 2023Review
- The Metabolism of Reactive Oxygen Species and Their Effects on Lipid Biosynthesis of Microalgae.International journal of molecular sciences · 2023Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide (H2O2) is recognized as an important signaling molecule in plants. We sought to establish a genetically encoded, fluorescent H2O2 sensor that allows H2O2 monitoring in all major subcompartments of a Chlamydomonas cell. To this end, we used the Chlamydomonas Modular Cloning toolbox to target the hypersensitive H2O2 sensor reduction-oxidation sensitive green fluorescent protein2-Tsa2ΔCR to the cytosol, nucleus, mitochondrial matrix, chloroplast stroma, thylakoid lumen, and endoplasmic reticulum (ER). The sensor was functional in all compartments, except for the ER where it was fully oxidized. Employing our novel sensors, we show that H2O2 produced by photosynthetic linear electron transport (PET) in the stroma leaks into the cytosol but only reaches other subcellular compartments if produced under nonphysiological conditions. Furthermore, in heat-stressed cells, we show that cytosolic H2O2 levels closely mirror temperature up- and downshifts and are independent from PET. Heat stress led to similar up- and downshifts of H2O2 levels in the nucleus and, more mildly, in mitochondria but not in the chloroplast. Our results thus suggest the establishment of steep intracellular H2O2 gradients under normal physiological conditions with limited diffusion into other compartments. We anticipate that these sensors will greatly facilitate future investigations of H2O2 biology in plant cells.
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