Evidence map›Paper›PMID 34195566›Full record

ArticleiScience2021

Human iPS cells engender corneal epithelial stem cells with holoclone-forming capabilities.

Shinya Watanabe, Ryuhei Hayashi, Yuzuru Sasamoto, Motokazu Tsujikawa, Bruce R Ksander, Markus H Frank, Andrew J Quantock, Natasha Y Frank, Kohji Nishida

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Shinya WatanabeDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Ryuhei HayashiDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
Yuzuru SasamotoDivision of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Motokazu TsujikawaDepartment of Biomedical Informatics, Osaka University Graduate School of Medicine, Division of Health Sciences, Suita, Osaka 565-0871, Japan.
Bruce R KsanderMassachusetts Eye and Ear, Schepens Eye Research Institute, Harvard Medical School, Boston, MA 02114, USA.
Markus H FrankTransplant Research Program, Division of Nephrology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Andrew J QuantockSchool of Optometry and Vision Sciences, Cardiff University, Cardiff CF24 4HQ, Wales, UK.
Natasha Y FrankDivision of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Kohji NishidaDepartment of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.
The University of Osaka · JPBrigham and Women's Hospital · USCardiff University · GBEdith Cowan University · AUMassachusetts Eye and Ear Infirmary · US

Funding

ABCB5-positive stem cells for LSCD therapyR01EY025794 · NEI · BOSTON CHILDREN'S HOSPITAL · PI NATASHA Y FRANK, Markus H. Frank · 2016 to 2026
$6.1M
Multipotent ABCB5-positive cell therapeutics for corneal diseaseR24EY028767 · NEI · BRIGHAM AND WOMEN'S HOSPITAL · PI FRANK, MARKUS H., FRANK, NATASHA Y · 2018 to 2022
$5.9M
NEI NIH HHS R01 EY025794NEI NIH HHS R24 EY028767
6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) can generate a multiplicity of organoids, yet no compelling evidence currently exists as to whether or not these contain tissue-specific, holoclone-forming stem cells. Here, we show that a subpopulation of cells in a hiPSC-derived corneal epithelial cell sheet is positive for ABCB5 (ATP-binding cassette, sub-family B, member 5), a functional marker of adult corneal epithelial stem cells. These cells possess remarkable holoclone-forming capabilities, which can be suppressed by an antibody-mediated ABCB5 blockade. The cell sheets are generated from ABCB5

Indexed as

BioengineeringCell biologyStem cells researchTissue engineering

Identifiers

PMID34195566
PMCPMC8233200
OpenAlexW3168456134

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.