ArticleFrontiers in cell and developmental biology2021
Complete Chromatin Decondensation of Pig Sperm Is Required to Analyze Sperm DNA Breaks With the Comet Assay.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Evaluation of Oxidative Stress in Mammalian Spermatozoa.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Embryo development is impaired by sperm mitochondrial-derived ROS.Biological research · 2024Article
- Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction.Journal of animal science and biotechnology · 2023Article
- An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm.Biological research · 2023Article
- Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.Journal of animal science and biotechnology · 2022Article
- Screen identifies fasudil as a radioprotector on human fibroblasts.Toxicology research · 2022Article
- Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs.Biological research · 2022Article
- Telomere Length in Pig Sperm Is Related to In Vitro Embryo Development Outcomes.Animals : an open access journal from MDPI · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm quality is usually evaluated prior to artificial insemination in farm animals. In addition to conventional semen analysis, other biomarkers, such as mitochondrial activity, integrity and lipid disorder of plasma membrane, generation of reactive oxygen species (ROS) and sperm DNA integrity, have been found to be related to fertility rates in different species. While mounting evidence indicates that the Comet assay is a sensitive method for the detection of DNA breaks, complete sperm chromatin decondensation is required in order to properly analyze the presence of single- and double-strand DNA breaks. In this sense, a previous study showed that longer lysis treatment with proteinase K is needed to achieve complete chromatin decondensation. The current work sought to determine which specific lysis treatment leads to complete chromatin decondensation in pig sperm, as this is needed for the measurement of DNA damage in this species. With this purpose, incubation with a lysis solution containing proteinase K for 0, 30, and 180 min was added to the conventional protocol. The impact of the DNA damage induced by hydrogen peroxide (H
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