ArticleChemical science2021
Quantification and molecular imaging of fatty acid isomers from complex biological samples by mass spectrometry.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Lipidomic Insight Across 48Microorganisms · 2026Article
- Recent advances in the analysis of unsaturated fatty acids in biological samples based on chemical derivatization and mass spectrometry techniques.Journal of pharmaceutical analysis · 2026Review
- Review
- On-Tissue Chemical Derivatization for Mass Spectrometry Imaging of Fatty Acids with Enhanced Detection Sensitivity.Biomolecules · 2025Article
- Unraveling the complexity of glycosphingolipidome: the key role of mass spectrometry in the structural analysis of glycosphingolipids.Analytical and bioanalytical chemistry · 2024Review
- Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies.Analytical and bioanalytical chemistry · 2024Review
- Review
- Dual Metal Electrolysis in Theta Capillary for Lipid Analysis.International journal of mass spectrometry · 2023Article
- Chemical tagging mass spectrometry: an approach for single-cell omics.Analytical and bioanalytical chemistry · 2023Review
- Single-cell lipidomics enabled by dual-polarity ionization and ion mobility-mass spectrometry imaging.Nature communications · 2023Article
- A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids.Biomolecules · 2023Review
- Mass spectrometry imaging: the rise of spatially resolved single-cell omics.Nature methods · 2023Article
- Circular RNAs in ferroptosis: regulation mechanism and potential clinical application in disease.Frontiers in pharmacology · 2023Review
- Chiral derivatization-enabled discrimination and on-tissue detection of proteinogenic amino acids by ion mobility mass spectrometry.Chemical science · 2022Article
- Characterization of glycerophospholipids at multiple isomer levelsThe Analyst · 2022Article
- Recent Advances in Mass Spectrometry-Based Structural Elucidation Techniques.Molecules (Basel, Switzerland) · 2022Review
- Reactive Thread Spray Mass Spectrometry for Localization of C═C Bonds in Free Fatty Acids: Applications for Obesity Diagnosis.Analytical chemistry · 2022Article
- Site-Specific Photochemical Reaction for Improved C=C Location Analysis of Unsaturated Lipids by Ultraviolet Photodissociation.Research (Washington, D.C.) · 2022Article
- Review of Recent Advances in Lipid Analysis of Biological Samples via Ambient Ionization Mass Spectrometry.Metabolites · 2021Review
- Advances in mass spectrometry imaging for spatial cancer metabolomics.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Elucidating the isomeric structure of free fatty acids (FAs) in biological samples is essential to comprehend their biological functions in various physiological and pathological processes. Herein, we report a novel approach of using peracetic acid (PAA) induced epoxidation coupled with mass spectrometry (MS) for localization of the C[double bond, length as m-dash]C bond in unsaturated FAs, which enables both quantification and spatial visualization of FA isomers from biological samples. Abundant diagnostic fragment ions indicative of the C[double bond, length as m-dash]C positions were produced upon fragmentation of the FA epoxides derived from either in-solution or on-tissue PAA epoxidation of free FAs. The performance of the proposed approach was evaluated by analysis of FAs in human cell lines as well as mapping the FA isomers from cancer tissue samples with MALDI-TOF/TOF-MS. Merits of the newly developed method include high sensitivity, simplicity, high reaction efficiency, and capability of spatial characterization of FA isomers in tissue samples.
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Registered trials
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