Trial reportScientific reports2021
Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients.
Trial report in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 36 citations in OpenAlex.
- Profiling of human and microbial cell-free DNA reflects early host-pathogen interactions in sepsis.Frontiers in immunology · 2026Trial
- Kinetics of cell-free DNA in response to exogenous and exertional heating in healthy young men.European journal of applied physiology · 2026Trial
- Circulating cell-free DNA as a biomarker in systemic lupus erythematosus: A narrative review.Journal of translational autoimmunity · 2026Review
- Cell-free DNA, cytokine, and catecholamine levels did not differ between sexes under a matched body core temperature increase, despite females exhibiting a more rapid increase in rectal temperature.European journal of applied physiology · 2026Article
- Female endurance athletes show a reduced plasma cell-free DNA response to all-out and 3-h cycling compared to male counterparts.Physiological reports · 2026Article
- Development and Qualification of a Nipah Virus Glycoprotein-Specific IgG ELISA for the Assessment of Human Antibody Responses.Vaccines · 2026Article
- Assessing the exercise-related kinetics of circulating cell-free DNA, circulating tumour DNA, DNase I activity and cytokines in patients with solid tumours: A pilot study.Experimental physiology · 2026Article
- Circulating extracellular vesicle DNA (EV-DNA) and cell-free DNA in ovarian cancer: pathological relevance and diagnostic applications.Extracellular vesicles and circulating nucleic acids · 2026Review
- Prospective observational study of cell-free DNA as a prognostic biomarker in COVID-19 and bacterial sepsis: COVSEP-study.Scientific reports · 2025Observational
- A Portable Fluorometer Detects Significantly Elevated Cell-Free DNA in Tracheal Wash and Bronchoalveolar Lavage Fluid in Horses with Severe Asthma.Animals : an open access journal from MDPI · 2025Article
- CfDNA as a surrogate marker for COVID-19 severity in patients with influenza-like symptoms with and without SARS-CoV-2 infection in general practice: a prospective cohort study.Virology journal · 2025Article
- Acute and Chronic Effects of a High-Intensity Interval Training Shock Microcycle on Cell-Free DNA: A Randomized Controlled Trial.Sports medicine - open · 2025Article
- Pre-analytical pitfalls: How blood collection tubes influence exercise-induced cell-free DNA concentrations.Experimental physiology · 2025Article
- Multimodal Web-Based Telerehabilitation for Patients With Post-COVID-19 Condition: Protocol for a Randomized Controlled Trial.JMIR research protocols · 2025Article
- Methodological influences on circulating cell-free-mitochondrial and nuclear DNA concentrations in response to chronic stress.Molecular biology reports · 2025Article
- Cell-free DNA release following psychosocial and physical stress in women and men.Translational psychiatry · 2025Article
- Exploring sex differences in blood-based biomarkers following exhaustive exercise using bioinformatics analysis.Biology of sport · 2024Article
- Predicting Injury and Illness with Machine Learning in Elite Youth Soccer: A Comprehensive Monitoring Approach over 3 Months.Journal of sports science & medicine · 2023Article
- Kinetics of Plasma Cell-Free DNA under a Highly Standardized and Controlled Stress Induction.Cells · 2023Article
- Observational
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circulating cell-free DNA (cfDNA) has been investigated as a screening tool for many diseases. To avoid expensive and time-consuming DNA isolation, direct quantification PCR assays can be established. However, rigorous validation is required to provide reliable data in the clinical and non-clinical context. Considering the International Organization for Standardization, as well as bioanalytical method validation guidelines, we provide a comprehensive procedure to validate assays for cfDNA quantification from blood plasma without DNA isolation. A 90 and 222 bp assay was validated to study the kinetics of cfDNA after exercise in patients with systemic lupus erythematosus (SLE). The assays showed ultra-low limit of quantification (LOQ) with 0.47 and 0.69 ng/ml, repeatability ≤ 11.6% (95% CI 8.1-20.3), and intermediate precision ≤ 12.1% (95% CI 9.2-17.7). Incurred sample reanalysis confirmed the precision of the procedure. The additional consideration of pre-analytical factors shows that centrifugation speed and temperature do not change cfDNA concentrations. In SLE patients cfDNA increases ~ twofold after a walking exercise, normalizing after 60 min of rest. The established assays allow reliable and cost-efficient quantification of cfDNA in minute amounts of plasma in the clinical setting. Additionally, the assay can be used as a tool to determine the impact of pre-analytical factors and validate cfDNA quantity and quality of isolated samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.