ArticleHorticulture research2021
An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 29 citations in OpenAlex.
- Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing.Plant direct · 2026Article
- Identification and molecular analysis of the Y-specific CpMp gene controlling long male peduncles in papaya.Nature communications · 2025Article
- Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants.BMC plant biology · 2025Article
- Characterization of an unusual carlavirus-like RNA from papaya (Carica papaya) lacking essential genes.PloS one · 2025Article
- Smartphone image dataset to distinguish healthy and unhealthy leaves in papaya orchards in Bangladesh.Data in brief · 2024Article
- Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC).Plant methods · 2024Article
- A Nimble Cloning-compatible vector system for high-throughput gene functional analysis in plants.Plant communications · 2023Article
- Proteome expansion in the Potyviridae evolutionary radiation.FEMS microbiology reviews · 2022Review
- Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).Plant methods · 2022Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Papaya (Carica papaya L.) is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced. However, functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species. Here, we developed a highly efficient virus-induced gene silencing (VIGS) vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus (PLDMV; genus: Potyvirus), PLDMV-E, into a stable Nimble Cloning (NC)-based PLDMV vector, pPLDMV-NC, in Escherichia coli. The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps. Using this PLDMV VIGS system, we silenced and characterized five endogenous genes in papaya, including two common VIGS marker genes, namely, phytoene desaturase, Mg-chelatase H subunit, putative GIBBERELLIN (GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors; and the cytochrome P450 gene CYP83B1, which encodes a key enzyme involved in benzylglucosinolate biosynthesis. The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya.
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