ArticleMicrobiome2021
Candidate probiotic Lactiplantibacillus plantarum HNU082 rapidly and convergently evolves within human, mice, and zebrafish gut but differentially influences the resident microbiome.
Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Cross-cohort single-nucleotide-variant profiling of gut microbiota suggests a novel gut-health assessment approach.mSystems · 2023Pooled it
- Precise probiotic therapy: Advances, bottlenecks, and the road to microbiome-informed nutrition.Gut microbes · 2026Review
- Bio-functional Levilactobacillus brevis strains of feline origin: the potential probiotic food adjunct for companion animals.BMC microbiology · 2026Article
- Glycodeoxycholic acid synergizes with L-malic acid to upregulate the malolactic enzyme pathway to alleviate self-toxicity inApplied and environmental microbiology · 2026Article
- Harnessing a germ‑free mouse gut bioreactor for directed evolution of probiotics to combat non-alcoholic fatty liver disease.Nature communications · 2026Article
- Genome-wide association analysis reveals specialization to hosts and niches in multiple species of the Lactobacillaceae.The ISME journal · 2026Article
- Bacillus licheniformis B410 Alleviates Inflammation Induced by Lipopolysaccharide and Salmonella by Inhibiting NF-κB and IRF Signaling Pathways in Macrophages.Probiotics and antimicrobial proteins · 2025Article
- Advances in adaptive laboratory evolution applications forSynthetic and systems biotechnology · 2025Review
- Gut microbiota drives structural variation of exogenous probiotics to enhance colonization.Gut microbes · 2025Article
- Article
- Human microbiome acquisition and transmission.Nature reviews. Microbiology · 2025Review
- Evolutionary adaptation of probiotics in the gut: selection pressures, optimization strategies, and regulatory challenges.NPJ biofilms and microbiomes · 2025Review
- Probiotic colonization ofInfection and immunity · 2025Article
- Distinct Effects ofMicroorganisms · 2025Article
- Article
- Different intestinal ecological niches drive the divergent evolution of probiotics in the gut.ISME communications · 2025Article
- Targeted Screening ofFoods (Basel, Switzerland) · 2024Article
- Zebrafish Feed Intake: A Systematic Review for Standardizing Feeding Management in Laboratory Conditions.Biology · 2024Review
- Data-driven prediction of colonization outcomes for complex microbial communities.Nature communications · 2024Article
- Article
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Authors and funding
15 authors.
Funding
Abstract
backgroundImproving probiotic engraftment in the human gut requires a thorough understanding of the in vivo adaptive strategies of probiotics in diverse contexts. However, for most probiotic strains, these in vivo genetic processes are still poorly characterized. Here, we investigated the effects of gut selection pressures from human, mice, and zebrafish on the genetic stability of a candidate probiotic Lactiplantibacillus plantarum HNU082 (Lp082) as well as its ecological and evolutionary impacts on the indigenous gut microbiota using shotgun metagenomic sequencing in combination with isolate resequencing methods.
resultsWe combined both metagenomics and isolate whole genome sequencing approaches to systematically study the gut-adaptive evolution of probiotic L. plantarum and the ecological and evolutionary changes of resident gut microbiomes in response to probiotic ingestion in multiple host species. Independent of host model, Lp082 colonized and adapted to the gut by acquiring highly consistent single-nucleotide mutations, which primarily modulated carbohydrate utilization and acid tolerance. We cultivated the probiotic mutants and validated that these gut-adapted mutations were genetically stable for at least 3 months and improved their fitness in vitro. In turn, resident gut microbial strains, especially competing strains with Lp082 (e.g., Bacteroides spp. and Bifidobacterium spp.), actively responded to Lp082 engraftment by accumulating 10-70 times more evolutionary changes than usual. Human gut microbiota exhibited a higher ecological and genetic stability than that of mice.
conclusionsCollectively, our results suggest a highly convergent adaptation strategy of Lp082 across three different host environments. In contrast, the evolutionary changes within the resident gut microbes in response to Lp082 were more divergent and host-specific; however, these changes were not associated with any adverse outcomes. This work lays a theoretical foundation for leveraging animal models for ex vivo engineering of probiotics to improve engraftment outcomes in humans. Video abstract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.