ArticlePloS one2021
A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Beyond Earth: Recent Advancements in Microgravity Biomedical and Genetic Research in Saudi Arabia.International journal of molecular sciences · 2026Review
- Emerging Chemical and Biological Materials Technologies in the Extraplanetary Environment.Nano-micro letters · 2026Review
- Exploring the role of CRISPR in advancing space biotechnology: challenges and solutions for human survival beyond earth.Frontiers in bioengineering and biotechnology · 2026Review
- Pharmaceutical and biomedical challenges for crew autonomy in health preservation during future exploration missions.Communications medicine · 2025Review
- Preventive Human Genome Editing and Enhancement: Candidate Criteria for Governance.The Hastings Center report · 2024Article
- A fluorescence viewer for rapid molecular assay readout in space and low-resource terrestrial environments.PloS one · 2024Article
- Microbial applications for sustainable space exploration beyond low Earth orbit.NPJ microgravity · 2023Review
- Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight.Cell reports methods · 2022Review
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As we explore beyond Earth, astronauts may be at risk for harmful DNA damage caused by ionizing radiation. Double-strand breaks are a type of DNA damage that can be repaired by two major cellular pathways: non-homologous end joining, during which insertions or deletions may be added at the break site, and homologous recombination, in which the DNA sequence often remains unchanged. Previous work suggests that space conditions may impact the choice of DNA repair pathway, potentially compounding the risks of increased radiation exposure during space travel. However, our understanding of this problem has been limited by technical and safety concerns, which have prevented integral study of the DNA repair process in space. The CRISPR/Cas9 gene editing system offers a model for the safe and targeted generation of double-strand breaks in eukaryotes. Here we describe a CRISPR-based assay for DNA break induction and assessment of double-strand break repair pathway choice entirely in space. As necessary steps in this process, we describe the first successful genetic transformation and CRISPR/Cas9 genome editing in space. These milestones represent a significant expansion of the molecular biology toolkit onboard the International Space Station.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.