ArticleAmerican journal of physiology. Cell physiology2021
Skeletal muscle progenitors are sensitive to collagen architectural features of fibril size and cross linking.
Article in American journal of physiology. Cell physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Treating age-related loss of muscle mass and function: Where should we be focusing?The Journal of physiology · 2026Review
- Mechanistic effects of percutaneous needle fasciotomy combined with stretching on early-stage skeletal muscle fibrosis in rats: focus on the TGF-β1/Smad signaling pathway.Journal of orthopaedic surgery and research · 2025Article
- A novel protocol for the direct isolation of a highly pure and regenerative population of satellite stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Muscle satellite cells and fibro-adipogenic progenitors from muscle contractures of children with cerebral palsy have impaired regenerative capacity.Developmental medicine and child neurology · 2025Article
- Integrating Physical and Biochemical Cues for Muscle Engineering: Scaffolds and Graft Durability.Bioengineering (Basel, Switzerland) · 2024Review
- Strain-dependent dynamic re-alignment of collagen fibers in skeletal muscle extracellular matrix.Acta biomaterialia · 2024Article
- Collagen architecture and biomechanics of gracilis and adductor longus muscles from children with cerebral palsy.The Journal of physiology · 2024Article
- Hierarchical Design of Tissue-Mimetic Fibrillar Hydrogel Scaffolds.Advanced healthcare materials · 2024Review
- Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue.ACS biomaterials science & engineering · 2024Article
- Senescent fibro-adipogenic progenitors are potential drivers of pathology in inclusion body myositis.Acta neuropathologica · 2023Article
- Extracellular matrix contribution to disease progression and dysfunction in myopathy.American journal of physiology. Cell physiology · 2023Review
- Thrown for a loop: fibro-adipogenic progenitors in skeletal muscle fibrosis.American journal of physiology. Cell physiology · 2023Review
- Insights into Intramuscular Connective Tissue Associated with Wooden Breast Myopathy in Fast-Growing Broiler Chickens.Foods (Basel, Switzerland) · 2023Article
- Tissue engineering modalities in skeletal muscles: focus on angiogenesis and immunomodulation properties.Stem cell research & therapy · 2023Review
- Spent media analysis suggests cultivated meat media will require species and cell type optimization.NPJ science of food · 2022Article
- A glitch in the matrix: the pivotal role for extracellular matrix remodeling during muscle hypertrophy.American journal of physiology. Cell physiology · 2022Review
- Synthesis of Fibrin-Type I Collagen Biomaterials via an Acidic Gel.Molecules (Basel, Switzerland) · 2022Article
- RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth.iScience · 2022Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Muscle stem cells (MuSCs) are essential for the robust regenerative capacity of skeletal muscle. However, in fibrotic environments marked by abundant collagen and altered collagen organization, the regenerative capability of MuSCs is diminished. MuSCs are sensitive to their extracellular matrix environment but their response to collagen architecture is largely unknown. The present study aimed to systematically test the effect of underlying collagen structures on MuSC functions. Collagen hydrogels were engineered with varied architectures: collagen concentration, cross linking, fibril size, and fibril alignment, and the changes were validated with second harmonic generation imaging and rheology. Proliferation and differentiation responses of primary mouse MuSCs and immortal myoblasts (C2C12s) were assessed using EdU assays and immunolabeling skeletal muscle myosin expression, respectively. Changing collagen concentration and the corresponding hydrogel stiffness did not have a significant influence on MuSC proliferation or differentiation. However, MuSC differentiation on atelocollagen gels, which do not form mature pyridinoline cross links, was increased compared with the cross-linked control. In addition, MuSCs and C2C12 myoblasts showed greater differentiation on gels with smaller collagen fibrils. Proliferation rates of C2C12 myoblasts were also higher on gels with smaller collagen fibrils, whereas MuSCs did not show a significant difference. Surprisingly, collagen alignment did not have significant effects on muscle progenitor function. This study demonstrates that MuSCs are capable of sensing their underlying extracellular matrix (ECM) structures and enhancing differentiation on substrates with less collagen cross linking or smaller collagen fibrils. Thus, in fibrotic muscle, targeting cross linking and fibril size rather than collagen expression may more effectively support MuSC-based regeneration.
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